Synthetic peptide vaccine patent manual (full) - table position problem.

: Why didn't Professor Wu synthesize the
amino acids
of surface
antigens
and core antigens (T.B.Th1
)? And synthesize only some of the amino acid sequences of these tables and combine them? Such as: core antigen on the 18-27 amino acid sequence we all know is HbcAg's CTL table, that 141-151, 117-215... What's it all about?
: The HBV core C
gene
is the most conservative among subsypes, and of the more than 180 amino acids it encodes, the CTL table is mostly located in the 150th amino acid ( amino acid, AA) Previously, 18 to 27 AAs were HLA-A0201, 88-96 AAs were restricted by HLA-A11, and 141-150 AAs were HLA-Aw68 And HLA A31 molecules are limited, in addition to the 75th to 81st AA or 72 to 88th AA is a strong configuration type B cell
table, so the core antigen is a more ideal vaccine research target molecules . In previous HBV vaccine studies, most people used S-gene-coded proteins, supplemented by CpG, IL2 and other molecular adulations to try to achieve immunization purposes, but the effect is not
ideal. While the preS1 gene coding protein contains the location of HBV and liver cell membrane binding, and is quite conservative, liver cells, B cells and T cells can express the subject of its 21st to 47th bit AA, so the former S1
antibody
is in addition to the surface antibody is another important mediated antibody.
: New Strategies for Autoimmune Disease Research: Reverse
Immunology
Wu Yuzhang Third Military Medical University 2007-7-19 13:13:07
Immunology and Reverse Immunology Research Advances, enabling us to start from the gene sequence to find disease-related antigens, disease-related immune responses, possible interventions and intervention strategies, and even interventions.
in autoimmune diseases, endologic red flags initiate an immune response or alter the immune network's stress and dynamics, leading to immune damage.
, understanding the mechanisms and interactions between the immune system and the "non-immune system" is the basis for understanding autoimmune diseases. Immunology and reverse immunology recognize and study this problem from the perspective of immune group-disease antigen group interaction.
previous research model was to look for a table from an antigen, and we asked the question "how to construct a simulated antigen to adjust the immune response to immunotherapy by the memogen".
introduced molecular design theory and technology into immunology, carried out immunological research in the new field of immunological identification, molecular design and antigen engineering, established a viral protein epitope database and in silico epitope prediction series methods, experimental detection series methods;
Based on the above theory and technology, a high-resolution immunometric identification study was carried out on the series
protein
antigen, and the series of tumor-related antigens and 55 dominant, protective and analog tables were identified, and the "non-soluble CTL table" (is this the legendary "new CTL effect mechanism"?) And the superantigen protope, found the heterogeneity of the CTL table, put forward a new view of "CTL table can be classified according to function", and put forward a new identification theory in protein antigen recognition - "protein antigen immunometric identification amino acid cryptology theory."
published 64 articles in the International SCI Journal.
to validate the above theories and techniques, we conducted reverse therapeutic vaccine studies using hepatitis B as a model. A new strategy for vaccine research on "overcoming immune tolerance by simulating antigens (mimogen) for immunotherapy" is proposed.
strategy of reverse immunology provides a new entry point for the study of autoimmune diseases.
.