Takara shares mRNA vaccine research solutions

"Vaccine" is undoubtedly one of the hot words during the new crown epidemic

The study of injecting in vitro transcribed mRNA into animals has been reported as early as the 1990s

The Takara mRNA vaccine research solution provides a variety of experimental tools for mRNA vaccine research to meet the needs of different research stages such as in vitro synthesis, transfection and expression detection

Takara mRNA vaccine research solution

mRNA synthesis in vitro

The production of mRNA is mainly carried out by in vitro synthesis, in which the RNA polymerase uses DNA as a template for in vitro transcription to obtain mRNA

※Check the complete content of the solution through the "Takara Bio China official website" to learn about restriction enzymes, ribonucleotides and other product information

mRNA transfection

Before conducting animal experiments, transfecting mRNA into cells is one of the methods to initially verify whether the synthesized mRNA is effective

Xfect is a biodegradable nanoparticle polymer transfection reagent, which can provide lower cytotoxicity and high transfection efficiency.

Experimental example

Western Blot protein expression detection

Western Blot analysis is still one of the commonly used detection and analysis methods in laboratories, and it is widely used in the detection and analysis of target proteins

Takara provides a wealth of Western Blot experiment related reagents, combined with detailed experimental operation guides, to improve the efficiency of Western Blot experiments

HPR substrate for chemiluminescence detection

Antigen-antibody reaction promotion

HPR-identified secondary antibody replacement

Blocking and elution

Easy to install electrophoresis buffer and protein Marker

"Western Blot Experiment Operation Guide", welcome to download and read
.

Viral RNA detection

Animal experiments are an important part of testing the effectiveness of vaccines
.
Among them, the detection of viral load in different experimental groups of animals is indispensable
.
In addition to classic histopathology and immunohistochemistry methods, fast and sensitive fluorescence quantitative PCR methods are also indispensable
.
Takara provides high-quality viral nucleic acid detection related products to accelerate the research process
.

Probe Method One-step RT-PCR Reagent and Viral Nucleic Acid Extraction

[Document Case]

-Huang, Qingrui, et al.
“A Single-Dose mRNA Vaccine Provides a Long-Term Protection for HACE2 Transgenic Mice from SARS-CoV-2.
” Nature Communications, vol.
12, no.
1, 2021, pp.
776– 776.

-Zhang, Na Na, et al.
"A Thermostable mRNA Vaccine Against COVID-19.
" Cell, vol.
182, no.
5, 2020, pp.
1271–1283.

The development of a vaccine is a race against the virus
.
mRNA vaccines are also steadily moving forward with ongoing related research.
Takara vaccine research program provides more options for the majority of vaccine research teams
.
Click on the topic below to learn more about genetic engineering vaccine research solutions
.

"Recombinant Adenovirus Vaccine Research Solution"

"Recombinant Protein Vaccine Research Solution"

Takara is open to various forms of business cooperation.
Sincerely welcome you to contact us about business cooperation.
Please send an email to Bulkorder@takarabiomed.
com.
cn

references

Ÿ Wolff, Jon A.
, et al.
“Direct Gene Transfer into Mouse Muscle in Vivo.
” Science, vol.
247, no.
4949, 1990, pp.
1465–1468.

Ÿ Pardi, Norbert, et al.
“mRNA Vaccines — A New Era in Vaccinology.
” Nature Reviews Drug Discovery, vol.
17, no.
4, 2018, pp.
261–279.

For more details, please click:
Takara Bio China official website