[Technology Daily] Gene editing "pipeline" helps prevent and control crop diseases

(Photo courtesy of Visual China)

The Xie Kabin research group of Huazhong Agricultural University has developed a set of gene editing "pipeline" called "FLASH", which not only optimizes the entire gene editing process, but also can be used to quickly identify the "identity" of gene editing materials and help identify the Genes related to important agronomic traits such as disease resistance, stress resistance, and yield

Recently, a research paper published by Professor Xie Kabin’s group at the State Key Laboratory of Crop Genetic Improvement of Huazhong Agricultural University and Hubei Hongshan Laboratory in "Molecular Plants" reported a large-scale, high-throughput method for editing plant genes.

The gene editing process is efficiently optimized

Gene editing technology has great potential in basic life science research and crop genetic improvement, so it has become a research hotspot in the life science field and the commanding height of scientific research competition in recent years

With the CRISPR/Cas9 gene editing technology becoming more and more mature in plant research, it is possible to use CRISPR libraries for high-throughput genetic screening

Plant CRISPR/Cas9 gene editing technology is more and more widely used in the agricultural field

Xie Kabin is one of the first researchers to develop plant CRISPR/Cas9 gene editing tools.

On the basis of the preliminary tools, Xie Kabin's research group created a gene editing material targeted to knock out 1072 rice receptor-like kinases, providing new resources for the rapid identification of genes related to disease resistance and stress resistance

Xie Kabin introduced that the "pipeline" includes computer programs for designing editing sites, high-efficiency CRISPR/Cas9 gene editing vectors, and high-throughput cloning methods for constructing gene editing vectors

"Carpet" search for specific genes in crops

Xie Kabin said that after loading the tag, the information of CRISPR/Cas9 vector and gene-edited plant materials can be read quickly and in real time through simple PCR

Thanks to the gene-edited library, researchers can perform a "carpet->

Developed a high-throughput gene editing "pipeline", and Xie Kabin's research group used this technology for rice disease resistance research

The research team discovered in experiments that a type of gene called receptor kinase is an essential component for plants to recognize pathogens

After conducting preliminary tests on receptor kinase-related genes, Xie Kabin's group selected 15 genetic materials for the rice blast inoculation test and identified 9 genes related to rice blast resistance

Xie Kabin said that gene editing is the most important application of the CRISPR/Cas9 system

Xie Kabin believes that these tools modified from the CRISPR/Cas system will greatly accelerate our basic research on crop diseases, and will also bring new technologies and new methods for green prevention and control of crop diseases

Original link: http://digitalpaper.