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(6) High performance liquid chromatography (HPLC)
HPLC has high sensitivity and specificity, and can be used for the analysis of TCs residues in animal blood, muscle, liver, kidney and other samples
.
Since TCs are easily adsorbed irreversibly with the remaining silanol groups on the reversed-phase silica gel column, leading to tailing of the chromatographic peak, it is usually necessary to add various tailing agents such as oxalic acid , phosphoric acid , citric acid , formic acid and hydrogen phosphate to the mobile phase.
1) Ultraviolet detection (UVD)
TCs have good UV absorption characteristics and a large molar absorption coefficient
.
Their maximum ultraviolet absorption wavelength in acidic solutions is generally between 350 and 380 nm, and near this wavelength range, compounds in body fluids and tissues rarely absorb, so there is less interference and can improve sensitivity
Samanidou et al
.
detected MNC, OTC, TC, CTC, and DC residues in beef by HPLC-DAD .
Zhang Suxia and others established an HPLC-UVD analysis method for TCs residues in milk
.
The samples were processed with MSPD technology, methanol acetonitrile-0.
Vias et al.
used reversed-phase HPLC-UVD to determine TCs in honey
.
It was extracted with a weak acid solution (containing EDTA to release the protein and sugar-bound drugs), purified by phenyl SPE column, and determined by HPLC-UVD
Related Links: Tetracycline Residue Analysis Technology-Determination of Thin Layer Chromatography
