The 19th photons are used to detect RNA concentrations.

to use the company's
. DNA
/RNA concentration meters to determine the values of samples A260 and A280. The RNA mass is estimated based on the A260/A280 ratio. The general A260/A280 ratio is between 1.8 and 2.0, which can meet the experimental requirements. Take 1 μl diluted to 250 μl (DEPC treated water) in 100 μl RNA original liquid, determine the value of A260 and A280 of the sample, and calculate its concentration according to the following formula: RNA raw fluid concentration: A260× dilution multiplied×4 The determination of the RNA concentration of 0 (ng/μl)
is carried out as follows:
(1) Take 1 μl of RNA raw liquid, place it in a 0.5 ml EP tube, add 249 μl dd H2O, mix it repeatedly with a pipet gun.
(2) adjust the A260 zero point with dd H2O as a blank control.
(3) pour out the water from the color cup, add diluted and mixed even RNA samples, and determine the A260 value. Pour out the RNA sample in the color cup, rinse the color cup with dd H2O, and adjust the A280 zero point.
(4) pour out the water in the color cup, add diluted and mixed even RNA samples to determine the A280 value. The A260/A280 ratio is calculated with a ratio of ≥.8 to meet the experimental requirements.
(5) RNA primary liquid concentration of A260× dilution multiply× 40 (ng/sl) .