The adaptive change pattern of protein temperature in marine molluscs is discussed.

Dong Yunwei's team's research on changes in protein temperature adaptability was published again in the Proceedings Journal of the National Academy of Sciences (PNAS) on December 24th, in collaboration with Professor George Somero of Stanford University's National Key Laboratory for Marine Environmental Science, The School of Marine and Earth Studies, and Professor George Somero of Stanford University. The National Academy of Sciences of the United States of America (The Proceedings of the National Academy of Sciences) published a research paper entitled "Comparing mutagenesis and simulations as tools for the research paper of the functional lyse ar truss for the protein thermal seiber", The adaptive change pattern of protein temperature in marine molluscs is discussed.
this article, in conjunction with the team's article "Rr flexibility and protein adapt to molecular: dynamics analysis of malate dehydrogenases of marine mollusc" published in early 2018 in PNAS, opened the way for the development of shellfish using computational biology.
Dong Yunwei's team, combining molecular dynamics simulation and experimental regulation methods during the study of the intertidal zone biobiochemical adaptation mechanism, found that at extreme high temperatures, heat-resistant binos can avoid protein chains, maintain microstructure integrity and function maintenance by enhancing the role of metabolic key enzymes, maintaining microstructure integrity and function maintenance (J Exp Bio l, 2017), through the study of 12 molluscs with a temperature span of 60 degrees C, the degree of temperature adaptability change of the flexible cMDH structure was quantified, the key sites of the evolution of amino acid temperature adaptability were revealed, and the intrinsic relationship between protein structure stability and biogeographic distribution was expounded.
expanded the study of biochemical adaptation of marine molluscs from a single qualitative experiment to quantitative research based on computational biology, revealed the convergence evolution model of the structural stability and functional adaptability of the marine mollusc cytoplasm malic dehydrogenase (cMDH), and established an innovative research model of biochemical adaptation mechanism based on the "enzyme-kinetic dynamics-protein synthesis-simulation calculation" of the metabolism of key enzymes.
Based on the above-mentioned basis, the research team further expanded the depth and breadth of the research, compared and analyzed the temperature tolerance of 26 kinds of marine molluscs cMDH, which can withstand high temperatures above 60 degrees C in Antarctica, and proposed the temperature adaptability change pattern of amino acids in different areas of protein, and revealed the region and its mechanism of action with important functions through molecular dynamics analysis.
series of research results to deepen the understanding of the temperature adaptation mechanism of marine organisms proteins, provide new research models and ideas in this field, for the identification of environmental temperature on biological distribution and its mechanism, to predict the ecological effects of climate warming is of great significance.
graph.(A) Ocean molluscs with different horizontal and vertical distributions and different in situ temperatures have different thermal tolerances and are negatively correlated with the degree of change in the rigidity and flexibility of cMDH structure; The change trajectory of 0-2ns structural degeneration; (C) the dipolymer structure of E.Malaccana, the red sphere is E. Malaccana, E.radiata, Littorina Keenae and L.scutulata cMDH non-conservative replacement sites, amino acid variation sites are always located outside the MRs, the four have a high sequence, but the thermal intolerance is very different.
the achievements were funded by the National Natural Science Foundation of China (Project Nos. 41776135, 41476115) and the Fujian Provincial Foundation for Distinguished Youth (2017J07003).
Dong Yunwei's team is devoted to the study of intertidal zone ecology, focusing on the response characteristics and temporal laws of intertidal organisms to complex environmental conditions, and their adaptation mechanisms. In recent years, the research of
has mainly focused on the mechanism of biobiochemical adaptation in the intertidal zone, physiological adjustment strategy and change of geographical pattern.
(1) Physiological regulation strategy: a physiological response model with energy metabolism and stress response as the main parameters was established, and the mechanism of multiple environmental stresss, such as temperature and precipitation, affecting population dynamics in the intertidal zone (Funct Ecol, 2016; Mol Ecol, 2014).
integrate environmental and physiological data to identify the sensitivity of intertidal organisms in China to temperature changes and their latitude characteristics (P Roy Soc B, 2017).
and foreign partners jointly published comments proposing to pay attention to the ecological effects of ocean multi-scale environmental changes (Nature, 2018).
(2) Changes in geographical pattern: it was determined that the biogeographic pattern of molluscs in China's intertidal zone, with the Yangtze Estuary as the boundary, and proposed that the construction of seawalls would lead to the migration of bio-distribution areas (Science, 2015);
Source: Xiamen University.