The apparent modification site and memory regulation network of plants' perceived springing signals.

The flowering of winter, second-year and perennial plants requires long-term ambient low-temperature induction, a process known as springing.
the discovery of the effect of springing has been nearly a hundred years.
With the progress of genetic and physiological research, it has been found that the role of springing is regulated by genetic and epigenetics, and plants have memory function in springification processing, but can only sustain it for a generation.
at present, researchers have some research on the apparent regulatory mechanism of springing effect, but only limited to a few genes, and lack understanding of other genes and the general change law of the springing regulatory pathway.
recently, the Chinese Academy of Sciences, the Chinese Academy of Sciences Institute of Plant Research fellow sage kang team through epigenetic analysis, revealed a new important control point in the role of springing epigenetic level VRN3, and excavated the molecular basis of the epigenetic memory of the role of springing.
researchers, using VRN1 as the known key gene for springization as a positive control, analyzed the dynamic regulation of two important histones modified H3K4me3 and H3K27me3 in springing by ChIP-Seq, and found that the H3K4me3 and H3K27me3 of vrN3 genes neglected in previous epigenetic studies showed significant changes, and the two complementeaching the gene in gene expression. further research
shows that VRN3 is the node of integrating two kinds of environmental signals, springing and light cycle, and it is also an important control point at the epigenetic level.
this result suggests that a variety of different regulatory signals are aggregated in this gene.
the study reveals the important regulatory points of the epigenetic level of springization and the regulatory network of epigenetic memory through genome-wide analysis, eliminating the deficiencies that have been confined to several key genes in previous studies.
the study identified genes that can maintain epigenetic modification changes in springing, and found that they are distributed in different physiological and biochemical pathways, indicating that epigenetic memory in springification not only controls flowering, but also regulates a variety of biological processes, so that plants are prepared for the development of nutrients into reproductive growth.
related research results published in the New Phytologist, The Ph.D. student of the Xiangkang team, Yu Qing, is the first author of the paper, and researcher Zhang Jingxuan and Xiangkang are co-authors.
the research was funded by the National Key Research and Development Program and the Chinese Academy of Sciences' China-Australia bilateral "CAS-CSIRO" project.
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