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    Home > Active Ingredient News > Drugs Articles > The application of lyophilized bead technique in the diagnosis of pet in vitro

    The application of lyophilized bead technique in the diagnosis of pet in vitro

    • Last Update: 2019-07-23
    • Source: Internet
    • Author: User
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    With the continuous improvement of people's living standards, the number of pet owners is also increasing rapidly According to the data, in 2016, the consumption scale of China's pet market reached 122 billion yuan, truly becoming a hundred billion yuan economy As people pay more and more attention to the health of pets, the scale of detection and diagnosis of pet conventional diseases is also growing rapidly in animal hospitals In the current clinical work of pets, there are a lot of problems that are difficult to diagnose or not able to diagnose some pathogens, such as: 1 The sensitivity of the existing diagnostic technology is low For example, although colloidal gold test paper can provide rapid diagnosis, there are certain cross reactions and animal autoantibodies interference in the detection of pathogens from the protein level, which lead to inaccurate diagnosis results in the process of clinical application ② The traditional diagnostic techniques (colloidal gold test paper and ELISA) can not provide accurate diagnostic basis for early diagnosis (such as incubation period) and diseases with high antigen requirements For example, canine distemper and parvovirus are common in clinic; ③ in the process of epidemic prevention and control, it is difficult to distinguish whether the antibody detected by serology is produced by vaccine immunity or virus infection; there are also some clinical pathogens that are difficult to diagnose when they are in small amount, such as diseases such as little BABEI PCR (polymerase chain reaction) PCR in vitro nucleic acid amplification detection technology has the outstanding advantages of specificity, sensitivity, high yield, rapidity, simplicity, good repeatability, easy automation, etc.; it can amplify the target gene or a DNA fragment to 100000 or even a million times in a few hours in a test tube, It enables the naked eye to observe and judge directly, and can amplify enough DNA from a hair, a drop of blood or even a cell for analysis, research, detection and identification In the past few days and weeks, you can do it in a few hours by PCR PCR technology is a revolutionary innovation and milestone in the field of biomedicine It has a wide range of application prospects in the field of pet diagnosis and human diagnosis Therefore, many biotechnology companies have begun to increase investment in PCR technology research and development PCR can detect pathogens PCR (especially real-time fluorescence quantitative PCR) makes the diagnosis of these diseases come true Relevant regulations have clearly listed virus nucleic acid detection (rather than test card detection) as the diagnostic standard of many animal diseases, which also shows that nucleic acid detection plays an important role in the diagnosis of animal diseases It is very important to choose a suitable PCR for pet diagnosis In clinical work, there are many PCR for veterinary doctors to choose After the reagent components of PCR are mixed, the liquid state PCR reagent will gradually degrade and fail at room temperature Therefore, there are problems such as short storage time, high cost of cold chain transportation, and inconvenient use With the appearance of freeze-dried beads, PCR technology has developed rapidly Liquid nitrogen, with its excellent characteristics (inert - no reaction with other substances), and reasonable price, determines that liquid nitrogen is an ideal low-temperature freezing medium The test reagent is quickly frozen into a small ball in liquid nitrogen through professional equipment, and the water inside the reagent small ball can be completely sublimed by vacuum freeze-drying technology without damaging the effective components After freeze-drying, the reagent turns into a solid ball state, which can be stored in a solid state at room temperature without cold chain transportation The product storage cycle is greatly extended, and the logistics cost is greatly reduced When used, the solid-state pellets are highly re soluble and can be dissolved quickly when meeting the sample liquid Then, it is the development trend of PCR reagent treatment at present, just like normal PCR reagent IDEXX company is a well-known multinational diagnostic company which is at the forefront of the application of liquid nitrogen freeze-drying bead technology
    With the increasing demand of pet market, many pet diagnostic reagent companies are committed to improving the convenience of in vitro diagnostic products At present, many companies will choose to make the diagnostic reagent into the shape of lyophilized beads and put it into the designed reagent disk after lyophilization The operator can simply operate according to the requirements, which greatly facilitates the rapid diagnosis and use of users However, at this stage, it is a major technical difficulty for pet diagnostic reagents to freeze-dried beads, how to trace the solution, product consistency, and production speed 1 Micropoint solution: the production process of pet diagnostic reagent lyophilized beads and pellets requires micropoint solution The common amount of micropoint solution ranges from 1 μ L (1 mg) to dozens of μ L It is difficult for the pipette that people are familiar with to keep the point liquid stable and keep the same amount of point liquid in such a small range Due to the technical principle, the accuracy is not high, the outflow control is not good, and the human factors of the operators, it is easy to cause the shape and size of the small ball are not the same, the shape of the ball is not good, or the small ball is exploded into multiple microspheres when it falls on the surface of liquid nitrogen 2 Precision spot solution: pet diagnostic reagent requires high precision and shape of lyophilized ball In laboratory operation, due to the problem of artificial consistency and simple equipment, it is difficult to control the accuracy and spherical consistency When the amount of liquid is small, it can't form a ball with the same shape When the amount is large, the lyophilized ball is difficult to re dissolve If the shape and size of the finished product lyophilized ball are different, the subsequent detection process and results will be affected 3 Industrialized low-cost mass production Traditional manual production, because of low efficiency, human factors affecting the consistency and stability of products, coupled with the growth of labor costs, is difficult to achieve low-cost mass production In order to solve the above problems, the technical team of Guangzhou feisheng precision equipment Co., Ltd began to focus on the research and development and manufacturing of liquid nitrogen freeze-drying bead point ball system since 2012, focusing on micro point liquid of micro level, breaking through the problem of poor precision, poor sphericity and uniformity of point liquid process in liquid nitrogen production process Based on the solid micro fluid pump technology, Guangzhou feisheng team has successively launched DS228 desktop system and DS328 floor system, which are suitable for laboratory / small batch production and large batch production of liquid nitrogen freeze-drying beads, with the volume as low as 1 μ L, and are in Taiwan, Famous bioengineering companies and biological research labs in Shenzhen, Chengdu, Tianjin, Ningbo, Zhuhai, Xiamen and other regions have provided system solutions for semi-automatic and automatic production of liquid nitrogen freeze-drying beads and become professional system suppliers in the application field of liquid nitrogen freeze-drying beads and beads The following is a brief introduction of two professional lyophilized bead point ball machines Ds228 desktop liquid nitrogen lyophilized bead production system ds328 floor standing liquid nitrogen lyophilized bead production system ds228 and ds328 liquid nitrogen lyophilized bead system adopt the precise micro ceramic pump technology with the point liquid volume as low as 1 μ L, and the components contacting with the liquid medicine are sapphire ceramic, Teflon and 316 Stainless steel materials, none of which will react with diagnostic reagents, thus ensuring the activity, stability and non pollution of reagents Ds228 and ds328 The volume and speed of the system can be quickly adjusted In addition, the company can customize all kinds of special needles, which can achieve the rate of liquid dispensing The liquid dispensing does not drop, and does not explode when it is put into liquid nitrogen The lyophilized bead ball has good circular effect, uniform shape and volume, and the same size, which effectively solves the problem that the lyophilized bead ball is easy to drop or explode, the accuracy is not accurate, and the ball forming effect is poor , poor consistency Guangzhou feisheng company has a variety of specifications of micro precision ceramic pump to choose from, as low as 1 microliter to more than dozens of microliters of liquid point range can achieve high-precision liquid point, liquid point accuracy as high as 0.5%, can meet the customer's various liquid point requirements Ds228 and ds328 system can realize automatic production The parameters of automatic production can be simply set through two screens The display 1 is the micro pump controller, which is mainly used to control various liquid point parameters of the micro pump Display 2 is the system operation panel, which is mainly used to control the overall parameters of the whole liquid nitrogen lyophilized bead system Ds228 and ds328 systems can be equipped with single channel or multi-channel micropump point liquid system Single channel micropump system is suitable for laboratory or small batch production, and multi-channel micropump system is suitable for batch production Ds228 and ds328 systems are designed with unique mechanism, which is convenient for quick collection and storage of lyophilized beads The system is equipped with integrated liquid nitrogen tank and optional safety liquid nitrogen filling device Ds228 and ds328 systems have the advantages of micro (as low as 1 μ L), high precision (0.5%), rate (100-200ppm), high consistency of lyophilized beads and balls, and automatic continuous production technology They are the systems of lyophilized beads and balls of biochemical companies Guangzhou feisheng precision equipment Co., Ltd is a high-tech enterprise specializing in precise fluid control system and solutions Its professional technical team serves well-known companies in the fields of BD, Guangbao biology, sannuo biology, Aikang biology, Tianjin micro nano core, Meikang biology and other bioengineering, pharmaceutical and medical devices, For China and Asia customers to provide point liquid, marking, spraying, filling and other micro fluid control system solutions
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