The basic knowledge of enzyme labeler and its detection principle.
Last Update: 2020-10-17
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is an electromagnetic wave with a wavelength of 100nm-400nm called ultraviolet light, light between 400nm-780nm can be observed by the eye, and a large son 780nm is called infrared light. People can only see color because the light shines on the object and is reflected back by the object. Green plants are green because they absorb the red spectral
is to detect the absorbent value of the measured object at a specific wavelength.
light passes through the detected object, the energy difference before and after the test is the energy absorbed by the detected object, at a specific wavelength, the concentration of the same detected object and the absorbed energy into a quantitative relationship.
detection unit is represented by an OD value, OD is an abbreviation for opticaldelnity (light density), which indicates the light density absorbed by the test, OD=10g (1/trans), where trans is the light transmission value of the detector. According to the Boober-amberT-beer law, the OD value is related to the light intensity as following:
E-OD-logI.0/I. Where E represents the absorbed light density, I.0 is the light intensity before the test, and I. is the light intensity from the detected object.
OD value is calculated by the following formula:
. E-OD× C×D
E: C is the concentration of the detector, D is the thickness of the detector, and E is the molar factor.
determines that each substance has its own specific wavelength at a specific wavelength at which it can absorb the most light energy. If you select a different wavelength band, the detection results are inaccurate. Therefore, when determining the detector, we select a specific wavelength for detection, called measuring wavelength.
but each substance still has a certain degree of nonse specific absorption of light energy, in order to eliminate this nonse specific absorption, we choose a reference wavelength to eliminate this inaccuracy. At the reference wavelength, the absorption of the light of the detector is minimal. Detecting the difference between the absorbent values of wavelengths and reference wavelengths eliminates nonse specific absorption.
Anthos enzyme marker detection value calculation
instrument detector receives the light energy through the detected object, converted into a two-digit digital signal, the maximum is 4095. The instrument defines no light source under the transmission value of 0%, no detection of the light transmission value of 100%. In the actual detection, the light transmission value of the detector is between 0% and 100%.
the light transmission value is calculated as follows:
. T (Meas-Min) / (Max-Min)
where T is the light transmission value, Meas is the two-bit value of the detection, Min is the two-bit value detected at 0%, Max is the two-bit value detected at 100% Binary values, for example:
Anthos enzyme marker's central positioning
instrument will automatically center the enzyme marker hole, the center positioning is to eliminate the enzyme label hole bottom caused by the bump thickness unevenness brings the detection of inaccuracies. When testing each enzymatic marker, the instrument actually measures 35 points and selects the average of the middle 5 points as the OD value of the hole.
the reference channel of a
light source is used to calibrate the effects of voltage instability or lamp wear.
and other tips for the use of
for the assay of ELISA
and are widely used in a variety of laboratories, including clinical laboratories.
quality control is an important factor in reagent testing. Please follow the requirements of the reagent instructions for quality control.
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