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    Home > Biochemistry News > Biotechnology News > The building process of histoprotein H3K4me3 and HK27me3 modification during embryo development before implantation.

    The building process of histoprotein H3K4me3 and HK27me3 modification during embryo development before implantation.

    • Last Update: 2020-09-14
    • Source: Internet
    • Author: User
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    On September 15, Tongji University's Gao Shaorong Laboratory published an article online in Nature magazine entitled "Distinct features of H3K4me3 and H3K27me3 chromatin domains in pre-implantation embryos".
    for the first time from the genome-wide level revealed the development process of histogenesic H3K4me3 and HK27me3 modification in mouse pre-implantation embryos, and found that wide (broad) H3K4me3 modification played an important role in gene expression regulation during pre-implantation embryo development.
    It can be said that the research results for the first time established the mouse pre-implantation embryo development of histoproteins H3K4me3 and H3K27me3 modified map, and found a special pre-implantation embryo development of the metastases of the metastases of the metastases of the metastases of the metastase of the metastases of the metastases of the surface genetic regulatory mechanism opened a door.
    with the occurrence of sperm egg binding, the combination of two end-differentiated reproductive cells to form an all-powerful fertilized egg.
    , the genomes of the parent and parent sources are extensively genetically reshaped to meet the needs of embryonic development.
    changes in these surface modifications are key to embryonic genome activation and first cell lineal differentiation.
    after transcription of alloproteins directly regulates the activation and silence of gene expression.
    early studies, using antibody immunofluorescent staining, it was found that most histoprotein modifications changed significantly during the development of pre-implanted embryos.
    abnormal expression or absence of enzymes that regulate histogen modification can lead to abnormal embryo development and even the death of pre-implanted embryos.
    studies have shown that changes in histoprotein modification play an important role in early embryonic development.
    how these histogenes are distributed and changed in the genome in pre-implanted embryos, how these changes regulate the expression of embryonic genes, and how the fate of the first cells differentiates is unknown.
    best way to fully understand histogene modification changes is to use antibodies modified by specific histoproteins for chromosomal immunocostory and in combination with chIP-seq techniques for second-generation sequencing.
    because the number of cells in the pre-implanted embryo is small and difficult to obtain and culture, it is impossible to obtain the millions of cells required by traditional ChIP-seq technology.
    in this study, Professor Gao Shaorong's team used and improved the newly published ULI-NchIP (ultra-low-input micrococcal nuclease-based native ChIP) technology for low-start cells.
    used a very small number of cells to detect changes in hismologic H3K4me3 and H3K27me3 modifications at various stages of pre-implantation embryo development in mice, which corresponded to gene activation and silence, respectively, the first known systematic genomic-wide testing of histogenesic modification of pre-implanted embryos in mice.
    analyzing the detected data, they found that the establishment patterns of histoprotein H3K4me3 and H3K27me3 modifications were significantly different, that H3K4me3 modifications were established more rapidly and tended to be built in the promoter regions with higher CpG content and lower DNA methylation levels, while H3K27me3 modifications were established more slowly and tended to be established in the lower CpG initiator regions.
    most important finding in the
    study was that, through data analysis, it was noted that although H3K4me3 modification rarely appeared to be fully established and removed after the 2-cell period, the width of the H3K4me3 signal was constantly changing, and there were a large number of wide (-gt;5kb) H3K4me3 signals in the genomes of early embryos.
    this wide H3K4me3 signal is low in cell line and in ordinary somogene cells.
    Importantly, these wide H3K4me3 signals are closely related to the high expression of genes and the developmental destiny of cells, which indicates that in early embryos, modifications such as H3K27me3 have not yet been fully established, and that cell regulation of gene expression may have completely different metagenetic regulatory mechanisms, including relying on changes in the width of H3K4me3 modification to regulate gene expression.
    the study also found that the width of the H3K4me3 modification changes gradually during embryonic development, with few direct establishment and removal of the wide H3K4me3 modification, which allows the wide H3K4me3 modification to remain relatively stable. In this study and in previous studies, it has been found that wide H3K4me3 modification is more likely to appear on the important regulatory factors of a particular cell type, indicating that the presence of wide H3K4me3 modification allows these important regulatory factors in the embryo to maintain the stability of gene expression levels even with interference from the external environment.
    a recent study has also shown that the presence of strong histoprotein-modified signals is closely related to stable mRNA expression, and that some areas without traditional histoprotein-modified modifications can be quickly activated or inhibited.
    this, the wide H3K4me3 modification accurately regulates gene expression at various stages as an adjustable metaphysic modification in early embryonic development and may play an important role in more physiological processes.
    further analysis showed that the histogene demethylase Kdm5b played an important regulatory role in the change of H3K4me3 signal length, and knocking down Kdm5b led to the general extension of H3K4me3 signals in the genome, as well as the blocking of embryonic development.
    in addition, in the study of the priceless gene, the study found that the amount of the priceless gene in early embryos is small and unstable.
    Dr. Liu Xiaoyu of Professor Gao Shaorong's lab at Tongji University, Wang Chenfei, ph.D. student in Professor Zhang Yong's lab, and Dr. Wenqiang Liu and Ph.D. student Li Jingyi of Professor Gao Shaorong's lab are co-authors of this paper.
    other authors of this article include Dr. Li Wei of Professor Gao Shaorong's lab, technicians Ko Xiaochen and Zhao Yanhong, Dr. Chen Jiayu, Dr. Gao Haibo, and Mr. Wang Hong.
    Gao Shaorong, Associate Professor Gao Yawei and Professor Zhang Yong are co-authors of this paper.
    the study was funded by the National Natural Science Foundation of China, the Ministry of Science and Technology, the Shanghai Science and Technology Commission and the Zhangjiang National Independent Innovation Demonstration Zone, and was completed at Tongji University.
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