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Plant synthesis structure of more than 200,000 kinds of metabolites, of which more than 20,000 kinds of steroid metabolites.
these metabolites not only play an important role in plant growth and development and environmental adaptability, many triamcinolone metabolites are also the main active active ingredients of Traditional Chinese medicine, has a very high application value.
in the process of plant synthesis of triamcinolone metabolites, 2,3-oxidizing sacryloene cyclase (OSC) is the key enzyme for the formation of metabolic diversity, and can synthesize more than 100 kinds of conformations and structures of the three skeletons by catalyzing 2,3-oxidizing sabiotic.
however, it is not clear how OSC uses the same substratetor to produce conformations and structures of triteron compounds.
the study team of the Institute of Plant Research of the Chinese Academy of Sciences identified indica parker alcohol coenzyme (OsPS) in the early work, which can synthesize the four-ring triamcinol of the chair-boat-chair-type configuration.
team recently discovered that the enzyme encodes a new, multi-product OSC-Indicaalcohol-coenzyme (OsOS) in the direct homologous gene of indica.
OsOS is capable of synthesizing a new chair-and-half-chair-chair-type conformation of the main product of five-ring triterium inseminasaol and 12 different triterium compounds.
by analyzing the homologous genes and functions of two subspecies from rice and their wild proximity, the researchers found that the vast majority of indica and wild rice have the catalytic function of OsOS, which is a single-function triamcinolase newly produced by its near-wild rice during the domestication process of indica.
researchers used molecular evolution analysis, protein structure simulation, fixed-point mutation and catalytic function analysis to identify three key amino acid sites with important influence on the conformation and structural changes of the product from 46 polymorphic sites.
amino acid residues at these sites determine the product and its conformation by affecting the spatial direction of the fourth amino acid residue (Tyr 257).
the study found a number of potentially active new triamcinols skeletons, including indical, revealed the catalytic mechanism of the formation of ship-type and chair triamcinolone skeletons, and laid the foundation for the future use of synthetic biology to create specific triamcinase and produce commercialvalue triamcinols active compounds.
the research was published online March 12 in the international academic journal New Phytologist. Xue Zheyong, an associate researcher in the
Paint Koizumi Research Group, and Tan, a Ph.D. graduate, are co-authors of the paper, and Ren Osbourn, a professor at John Innes Centre in the United Kingdom, is a co-author of the newsletter.
the study was co-funded by the National Natural Science Foundation, the National Fund for Study Abroad, and the British Biotechnology and Biological Sciences Research Council (BBSRC).
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