The Chinese Academy of Thermal Sciences has made new progress in the study of the common mechanism of low temperature and drought response of bananas

Recently, the banana genetic improvement research team of the Institute of Biology of the Chinese Academy of Thermal Sciences/Haikou Station has made new progress in the study of the common mechanism of banana low temperature and drought response, confirming the molecular mechanism of MaDREB1F transcription factor conferring low temperature and drought stress resistance in bananas by co-regulating hormone synthesis and protective metabolite content, which provides a theoretical basis and excellent genetic resources
for banana stress resistance molecular breeding.

 

Bananas are extremely sensitive crops to low temperature and drought stress, and improving banana resistance to low temperature and drought stress is an urgent need
for industrial development.
In this study, aiming at the problem of unclear common mechanism of CBF/DREB1 transcription factor regulating plant response to low temperature and drought stress, it was revealed that MaDREB1F has nuclear localization characteristics and transcriptional activation activity through genetic transformation, transcriptome, yeast single hybridization and other technologies, and is significantly induced by low temperature and drought stress.
MaDREB1F improves the low temperature and drought stress resistance of bananas by regulating soluble sugar and proline levels, activating the antioxidant system, and promoting the synthesis of jasmonic acid and ethylene.
Under low temperature and arid conditions, MaDREB1F directly activated the promoter activities of MaAOC4, a key enzyme gene for jasmonic acid synthesis, and MaACO20, a key enzyme gene for ethylene synthesis, to promote the synthesis of jasmonic acid and ethylene.
Under drought conditions, MaDREB1F can target binding to MaERF11 promoter, activate MaACO20 expression, and promote ethylene synthesis
.
In conclusion, this study provides new insights into the common mechanism of CBF/DREB1-mediated response to low temperature and drought stress, and provides a theoretical basis
for using key modules of biotechnology modification to improve crop resistance to low temperature and drought stress.

 

The results of the study are "MaDREB1F co nfers cold and drought stress resistance through commonly regulating hormones synthesis and protectant metabolite co.
" ntents in banana" in Horticulture Research
.
Associate Researcher Xu Yi and Associate Researcher Song Shun of Haikou Experimental Station of Chinese Academy of Thermal Sciences are the first authors of the paper, and Professor Jin Zhiqiang, Researcher Xu Biyu and Researcher Hu Wei of the Institute of Biology of Chinese Academy of Thermal Sciences are the corresponding authors
of the paper.
The research has been supported
by the National Key Research and Development Program of China, the National Natural Science Foundation of China and other projects.

 

Article link: https://academic.
oup.
com/hr/advance-article/doi/10.
1093/hr/uhac275/6881686?searchresult=1