The composition and diversity of the biomass (-0.2 sm) community of the Xiamen water supply reservoir.

Plankton are a major component of the lake ecosystem and play a fundamental and critical role in material circulation, pollutant degradation and water self-purification.
reservoir micro-nuclear plankton (0.2-200 m) including prosthylobacters, algae, low-class fungi and small post-organisms, with small individuals, diverse species, wide distribution and so on.
a long time, the ecological research of micro-nuclear plankton community mainly relies on microscopic technology for observation, the problem is that it is difficult to identify and accurately identify similar species and extremely small individual species.
to avoid this problem, researchers have in recent years used 18S rDNA high-volume sequencing technology to obtain richer and more diverse information about futons, but this method does not directly distinguish between individual sizes.
practice for collecting micro-nuclear plankton is to use a hierarchical filtration method to distinguish between micro and large-scale nuclear plankton.
, however, the effectiveness of DNA sequencing analysis based on graded filter plankton still lacks quantitative assessment and systematic understanding.
Yang Jun's team from the Water Ecological Health Research Group of the Institute of Urban Environment of the Chinese Academy of Sciences compared the composition and diversity of the groups of two water supply reservoirs in Xiamen using 18S rDNA high-volume sequencing and 200 m aperture filter grading filtering.
results showed no significant changes in the abundance, diversity and 99.2% OTU abundance of the total nuclear plankton species after filtering through the 200 m aperture filter, and that both granular biomes contained 25% of the post-living animals (arthropod-based).
in-depth comparative analysis found that the futosic plankton community showed similar, synchronized patterns of space-time change, and the environmental factors that could explain the changes of the two granular biomes were exactly the same.
results are mainly due to the fact that the use of 200 m grading filtration is not effective in removing the larvae, residues and environmental DNA of large uterine plankton.
, the study also revealed the effects of sequence sequencing depth and quality control on plankton community analysis based on biometrics and statistical methods.
in general, dna high-volume sequencing based on 200 m grading filtration cannot avoid environmental DNA contamination, and future quantitative studies will need to combine RNA high-volume sequencing and microscopy techniques for comprehensive analysis.
research has been funded by the National Natural Science Foundation of China, Fujian Provincial Science Foundation for Distinguished Young People, and the Institute of Urban Environment of the Chinese Academy of Sciences.
related paper DNA metabarcoding reveals that 200 sm size-fractionated filtering is unable to peere between planktonic microbial and large eukaryotes has been published online in the international journal Molecular Ecology Resources.
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