The content of serum triglycerides was determined

related topics

The
objectives of
:1. Master the principles and methods for determining triglycerides.2. Understand
normal value
and clinical significance of serum serum triglycerides.The main physiological functions oftriglycerides are energy storage and energy supply, mainly distributed in fat
tissues such as the epithelral, abdominal large retina, intestinal membrane, and
internal organs. In the serum, endo-triglycerides are mainly transported in the form of VLDL, and exosomic triglycerides are mainly transported in the form of CM. In normal adults, the content of triglycerides was 0.11 to 1.69 (average 1.13). The blood lipid content is influenced by many factors, such as diet, race, sex, age, occupation, exercise status, physiological state and
hormone
level. For example, plasma cholesterol levels in young people are lower than in the elderly, and in some diseases, blood lipid levels vary significantly, such as diabetes and atherosclerosis. Therefore, it is of great clinical significance to determine the content of blood lipids.The triglycerides in the blood are taken in a joint ethane-isopropyl alcohol, and then koH saponified withdrawal is used to produce glycelin, and then sodium iodide oxidated glycelin is used to produce formaldehyde, and finally formaldehyde is reduced with acetyl acetone and ammonia to produce 3,5-diacetyl-1,4-dihydrodmethylamine with yellow fluorescence./>/>
equipment and
reagents 1. equipmenttest tube
and test tube rack, mixer,
thermostat
water bath, microscope, straw, ear-sucking ball, visible light
diluent meter
, color cup, marker pen.
2. Reagents(1) fresh blood.(2) The pumping agent is positive octane-isopropyl alcohol (2:3.5) (V/V).(3) 0.04mol/L H2SO4 solution.(4) Isopropyl alcohol.(5) Saponification reagent: 6.0g/> KOH dissolved in 60 ml distilled water, mixed with isopropyl alcohol 10 ml, placed in a brown bottle, room temperature preserved. (6) Oxidation reagents: 650 mg of sodium periodiodeate dissolved in about 100 ml distilled water, added 77g/> ammonium acetate, dissolved and then added into 60 ml of ice acetic acid, add water to 1000ml, in brown bottle room temperature preservation. (7) Acetyl acetone reagents: 0.4 ml of acetyl acetone plus isopropyl alcohol to 100 ml, placed in brown bottles, stored at room temperature. (8) Triglyceride standard liquid: precisely named triglyceride 1.00g/> in 100ml
capacity bottle
, add a suggle to the scale, this is the storage standard liquid (10g/>/L). When in use, dilute 10 times with a sugging agent, i.e. 1g/>/L application fluid, refrigerator storage.

the "Procedure" 1. Take 3 dry test tubes, number, add reagents accurately according to the table: reagents blank tubes standard tubes assay tubes serum (ml) - - 0.2 standard liquid (ml) - 0.2 - distilled water (ml)
0.2 -- pump (ml) 2 2 2 0.04mol/LH2SO4 (ml) 0.6 0.6 0.6 0.6 side-by-side shaking, gabi shaking 25min, then static layering. 2. Take 3 additional dry test tubes of the same number, each plus layer liquid 0.3 to 0.5 ml (three tubes of liquid equivalent), and then add isopropyl alcohol 2 ml and saponifying agent 0.4 ml, immediately shake well, in 65 degrees C / > insulation 5min. 3. Each tube is added to the oxidant 2 ml and acetyl acetone reagent 2 ml, fully mixed. To 65 degrees C/> water bath insulation 15min, with cold water cooling, 420nm wavelength or blue filter color, with blank tube correction zero point, read each tube absorbent. (Calculation) serum triglyceride mmol/L (measuring tube absorbance/standard tube absorbance) ×0.2×100÷0.2×0.011 Photons/standard tube absorbances) ×1.1 normal values: 0.22 to 1.21mmol/L Note1. Subjects of this test need to be on an empty stomach for 12 to 14 hours. 2. The absorption peak of acetyl acetone is around 415nm. 3. 0.11 in the calculation formula is the coefficient of mg/L converted to mmol/L. 4. This method uses cheap octane as a mixed suction agent with isopropyl alcohol. In addition, petroleum ethers such as octane or pyrane and petroleum ethers with a boiling range of 98.4 to 150.70 degrees C/> can be used. Therefore, the pump can also be used gasoline - isopropyl alcohol. What is the H2SO4 in this experiment?