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    Home > Biochemistry News > Microbiology News > The cultivation method of black tea bacteria

    The cultivation method of black tea bacteria

    • Last Update: 2020-06-21
    • Source: Internet
    • Author: User
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    , training methodsblack tea bacteria are made from black tea (or with green tea, oolong tea, bitter tea, etc.), white sugar (or iced sugar, honey) and waterIts cultivation methods are as follows:(1) the production of culture liquid: A, proportional tea, clean water, white sugar, B, tea with white gauze wrapped, C, water aluminum pot or steel pot boiled and sterilized; D, put tea bags into boiling water 2-3 minutes, fished out; E, put white sugar to melt; F, tea water cooling to 35 degrees below(2) inoculation culture: in a large disinfected glass bottle or porcelain cylinder culture, the mother liquid added to the appropriate amount of flushing the inner wall to drop, and then put good mother bacteria liquid, bacteria film added to the culture bottle, the cool culture liquid added to the incubator, with gauze sealing insect protection, and dustThe culture bottle sun without direct sunlight culture, high fermentation fast, low temperature fermentation slow, generally in 30 degrees to 35 degrees a week or so, the surface of the bacteria into a milky white fresh fungus membrane, culture liquid is fermented into sweet and sour black tea bacteria, can be consumedBlack tea bacteria liquid do not drink, leaving part of the bacteria liquid can also continue to be as the method of culture drink ingress, circulation is endlessThe proportion of raw materials for the cultivation of black tea bacteria is black tea: sugar: water: 0.2:5:200"text-align: "center":img src""alt""""black tea bacteria culture method" title"""ii, black tea bacteria culture precautions 1, do a good disinfection workThe most important thing is disinfection, culture of black tea bacteria containers must do a good job of disinfectionMethod 1, boil ingest for 10 minutes in boiling water, method 2, rinse with mother bacteria liquid for a second time(If the culture process found that the surface of the culture liquid has a black, green or red other flora must be abandoned)2, the choice of the speciesTo choose fresh bacteria and membrane, bacteria are lactic acid bacteria, acetic acid bacteria, yeast symbiosis, these bacteria are microorganisms, can only be seen under the microscope, the main presence of bacteria, the membrane is mainly acetic acid aging condensation into clumps, is the relic of acetic acid bacteria, can not be alone as a species of inoculation culture of red tea bacteria, bacteria can not be good or bad; The use of jars, glass bottles and other cultivation, avoid not being available for plastic, iron products cultureCan maintain the original black tea bacteria many excellent quality3, the choice of raw materialsIt is best to use some untreated natural green tea, black tea and other tea, sugar selection white sugar, less use of honey, honey impure, more impurities, water selection of clean well water, spring water, water with disinfectant disabled4, sugar, tea, water deliveryThe important ratio of sugar, tea and water is 5:0.1-0.4:100 (5 to 0.1 to 0.4 to 100)The first time the culture liquid to do less, the second experience can be doubled5, calm, ventilated, avoid direct sunlightBe sure to do a good job of ventilation to prevent other bacteria into life, ventilation is very important, because ventilation can effectively reduce the concentration of other bacteria in the culture apparatus so that other bacteria can not live6, when buying bacteria, should choose color health, fresh bacteria, the more the more the amount of the enemy can better resist the natural infection, so the success rate will be highertea bacteria floating is not dead, if the black tea bacteria die, it will sink, become smallerIf the black tea bacteria live, it will slowly become larger, good is white, yellow, yellow, red, green, not good, sugar water is to feed its nutrients, to change every day, a long time, will have an impact on the tea bacteria!
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