The development of domestic clenbuterol test kit

Introduction: according to the Institute of nutrition and food hygiene, Chinese Academy of preventive medicine, the development of domestic clenbuterol detection kit was successful According to reports, if every pig entering the market carries out the detection of clenbuterol with domestic reagents, the price of each kilogram of pork is only a penny more than the original price, which can greatly strengthen the detection of clenbuterol At present, the research group is using the traditional detection method to further calibrate experiments, which will soon be put into commercial production and practical application The newly developed method belongs to enzyme-linked immunosorbent assay (ELISA), which is based on antigen antibody reaction for competitive inhibition assay The detection sensitivity of reagent amount can reach 0.5 ppb At present, the detection of clenbuterol in pig urine by the Ministry of agriculture of China is based on the standard of 1 ppb, so it can completely meet the needs of supervision and detection standard The monoclonal antibody technology is used in the kit, which is better than foreign products in specificity The results also showed that enzyme-linked immunosorbent assay, as a screening method for clenbuterol, was simple, accurate and rapid, and suitable for the determination of a large number of samples J83