The effect of amber acid dehydrogenase and the observation of competitive inhibition.

related topics .(i) The original alumic acid
dhydrogenase
is an important enzyme in the triacetic acid cycle, and determining whether there is such an enzyme in the cell can initially determine the existence of the triacetic acid cycle pathway.acid dehydrogenase dehydrogenase dehydrogenates its substrate, producing hydrogen that can be produced by a series of conveyors that are finally handed oxygen.in the absence of oxygen, if there is a suitable hydrogen body can also show the role of dehydrogenase. Such as the heart muscle of the amber acid dehydrogenase in the absence of oxygen, can make the amber acid dehydrogenation to produce yanhuso acid, the hydrogen can be reduced to blue methylene blue into colorless toluene white.this way, the role of amber acid dehydrogenase can be shown.chemical structure of propylene disic is similar to that of amber acid, which competes with amber acid and binds to serum acid dehydrogenase. If the amber acid dehydrogenase has been combined with propylene disic, it can no longer catalyz the dehydrogenation of amber acid, a phenomenon known as competitive inhibition. If the concentration of serum acid is increased relatively, the inhibition of propylene acid can be reduced.
(ii) Reagents
1. 1.5% sodium sodium sephate solution: 1.5g sodium sodium sodium sephate, dissolved with distilled water and diluted to 100ml. If no sodium sodium sodium sephate can be used to form an aqueous solution, the solution of sodium hydroxide is mediumed to pH7 to 8.
2. 1% sodium propylene diphthate solution: 1g of sodium propylene diate, dissolved with distilled water and diluted to 100 ml.
3. 0.02% methylene blue solution.
4. 1/15mol/LNa2HPO4 Solution: Take Na2HPO4 2H2O 11.8g, dissolved with distilled water and diluted to 1,000ml.
5. Liquid paraffin.
(iii) Conducting
1. Pig heart preparation liquid: said to take fresh pig heart 1.5 to 2.0g put
tissue
masher, add equal volume of quartz sand and 1/15mol/LNa2H PO4 solution 3 to 4 ml, mashed into pulp, and then add 6 to 7 ml 1/15mol/LNa2HPO4 solution, placed for 1 hour, shake from time to time, centrifugation to take the liquid backup.
2. Take 4
tubes
, number and follow the table:







3. After mixing each tube solution, each tube is added a thin layer of liquid paraffin (about 5 to 10 drops). Why?
4. Each tube is placed in a water bath at 37 degrees C, and within half an hour observes the color changes of each tube, compares its speed and explains the reason. Then shake the 1st tube hard to see how it changes. Why?
.