The efficiency mechanism of the steady-state regulation of nitrogen fertilizer is revealed

fertilizer is one of the factors to promote crop yield. However, the increase in nitrogen fertilizer use in recent years has not led to a significant increase in crop yield, but the economic and ecological benefits have shown a downward trend. How to improve the efficiency of nitrogen fertilizer utilization has become an urgent problem in agricultural production. Cultivating new varieties of crops for efficient use of nitrogen fertilizer is an effective way to reduce production costs, reduce environmental pollution and greatly increase ecological benefits.
December 29, Plant Cells published a research paper by Li Shan, a professor at Nanjing Agricultural University, and Wang Shaokui, a professor at South China Agricultural University.
The study used "Huayu 74" as the background of the rice single-fragment replacement material, combined with overlapping group drawing and map cloning technology from indica isolated to nitrogen efficient use of the gene DNR1, the gene encodes acetaldehyde phosphoric acid-dependent amino transferase, negative regulation of growth hormone synthesis.
have been reported that growth hormone can regulate the efficiency of nitrogen fertilizer utilization in plants, but the specific molecular mechanism is not clear. The study found that the external nitrogen source can change the growth hormone content in rice by regulating the expression level of the DNR1 gene, thus affecting the ability of the growth hormone signaling pathway to respond to the activation of the gene OsARFs on the downstream nitrogen metabolism-related genes, and finally realizing the regulation of the efficiency of nitrogen fertilizer utilization in rice. The N-DNR1-Auxin-OsARF molecular module revealed in this study enriches the understanding of nitrogen-growth-nitrogen fertilizer utilization efficiency, and reveals the mechanism of growth hormone steady-state regulation of nitrogen fertilizer utilization efficiency from the molecular level.
DNR1's initiator sequence has a 520 bp difference between the subseeds of indica and rice, which leads to a significant difference in the absorption rate of nitrogen fertilizer between indica and indica, and the low expression of the DNR1indica allegan gene in indica leads to an increase in the growth hormone content in indica, which makes it more capable of nitrogen fertilizer utilization. The cultivation of indica varieties and DNR1 knock-out systems in large fields with different amounts of nitrogen fertilizer, DNR1 knock-out system can increase yield by 8% to 25%, which shows that DNR1 has great application potential and value in improving the utilization efficiency of indica nitrogen fertilizer. (Source: Wang Fang, China Science Journal)
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