The epigenetic characteristics of iPS cells, the source of chemical small molecules, are closer to ES cells.

Recently, Yao Hongjie, a researcher at the Guangzhou Institute of Biomedicine and Health research of the Chinese Academy of Sciences, worked with Jiang Zhizhong, a professor at Tongji University's School of Life Sciences and Technology, to reveal that the epigenetic characteristics of iPS cells from the source of chemical small molecules are closer to the epigenetic characteristics of ES cells.
embryonic stem cells (ES cells) have great potential for application in the clinical application of regenerative medicine, but they are limited because of ethical problems.
reported that epigenetic stem cells (4F-iPS cells) induced by using the classic four transcription factors Oct4, Sox2, Klf4 and c-Myc-induced results were shown to differ from ES cells. Deng Hongkui, a professor at Peking University
, was the first to use chemical small molecule-induced obtaining pluripotent stem cells (C-iPS cells), opening up a new method of iPS cell acquisition.
but the current study has less to know about the epigenetic characteristics of C-iPS cells.
in this study, Yao Hongjie's team studied the relationship between DNA methylation mapping and gene expression of C-iPS cells, 4F-iPS cells and mES cells, in cooperation with Jiang Shizhong's team on the basis of using chemical small molecules to induce fiber cells into C-iPS cells.
the researchers first obtained DNA methylation maps and gene dating data for C-iPS cells, 4F-iPS cells and mES cells under the same conditions through simple and sulphate sequencing (RRBS) and transcription group sequencing, respectively.
analysis found that although C-iPS cells, 4F-iPS cells and mES cells were almost as methylated at the genome-wide level, the first two were slightly more methylated than mES cells, and 4F-iPS cells had a higher degree of DNA methylation than C-iPS cells.
researchers found that the methylation state of the reverse transcription translate element of C-iPS cells was closer to the methylation state of the reverse translucent element of the mES cell than in 4F-iPS cells.
C-iPS cells have similar levels of DNA methylation and gene expression to the imprinting genes of mES cells, while many imprinted genes in 4F-iPS cells are silenced by abnormal LYD high methylation.
the study suggests that chemically small molecularly induced iPS cells may provide a better strategy for both basic research and future clinical applications, while transcription factor-induced acquisition of imprinted gene abnormal DNA methylation caused by iPS cells may be a limiting factor in future clinical applications.
research published in cell Death and Disease.
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