The experimental method and procedure of fluorescent quinometer to determine the concentration of sulfate quinine.
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Last Update: 2020-10-21
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Source: Internet
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Author: User
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related topics .Fluorescence analysis method has the characteristics of high sensitivity, strong selectivity and low sample amount. The main purpose of this experiment is to determine sulfate quinine by learning fluorescence, to master the use of VARIAN fluorescence
phosphorescometer
and to use fluorescence method for quantitative analysis.I. Experimental Purpose 1. Familiarize yourself with the principles of VARIAN fluorescence spectrophotometer and use 2. Master the concept and measurement method of excitation
spectral
and emission spectra Realization ofspectral scanning: theinstrument has two
grating
spectroscopic systems that can form two spectra in the form of excitation and emission grating motion:excitation spectrum: excitation grating rotationemission spectrum: emission grating rotation fluorescent material excitation spectrumfixed emission wavelength. The fluorescence intensity F emitted by
compound
is the relationship between the fluorescent intensity F and the incoming light wavelength of the fluorescent substance at different wavelengths, 2. Emission spectra (or fluorescence spectra)fixed excitation wavelengths. Fluorescence intensity of various wavelengths in the fluorescence emitted by the compound.. Second, the experimental principle 1. Molecular structural characteristics of sulfate quinine sulfate quinine is alkaloid anti-heart rate disorder drug, its molecule has aquinine ring structure, can produce strong fluorescence, can directly use fluorescence method to determine its fluorescence intensity, by correcting the curve to find the regression equation and then find the concentration of quinine in the sample.
.2. Quantitative basis and method (1) Quantitative basis:At low concentrations, the fluorescence intensity of the solution is linearly related to the concentration of fluorescent substances in the solution If s Kc(1) 2) Quantitative method: correction curve methodstandard curve method:preparation of a series of standard concentration samples to determine fluorescence intensity, drawing standard curve, and then under the same conditions to measure the fluorescence intensity of unknown samples, in the standard curve to find the concentration;III, Experimental Content .1. Instruments and
Reagents
:Instruments: Fluorescent phosphorescometer, scale
straw
,
capacity bottlesReagents: 0.1 μg/ml sulfate quinine (instrument room), 100 sg/ml sulfate quinine standard solution,Sulphuric acid quine liquid to be tested 0.05mol/l thin sulphuric acid solution .2. Experimental steps: 1. Preparation of standard solution:Precision absorption of sulfate quinine standard solution (100 μg/ml, 0.0.1 05 mol/L sulphuric acid dissolved) 1.0, 2.0, 3.0, 4.0 and 5.0 ml were placed in 50 ml capacity bottles, diluted to the line with 0.05mol/L sulphuric acid, shaken, made
. Control products
series solutions and calculate concentrations..2. Preparation of sample solution to be tested:absorb quinine sulfate to be tested 1.0 ml, in a 50 ml capacity bottle, diluted with 0.05mol/L sulfuric acid to the line, shake well, to be tested sample solution..3. Assay:(1) Determination of excitation spectra and emission spectra with 0.1ug/ml of sulfate quinine:power-on-open spectral scanning workstation-method-establishment-zero-excitation spectral assay-emission spectra determination-printing results (2) correction curve method to determine the concentration of the sample solution:Open concentration determination Workstation - method establishment - determination of correction curve - determination of sample solution - printing results - shutdownPrecautions: solution preparation process should be standardized operation of various capacity instruments to prevent errors
;
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