The feasibility of efficient production of flavonols using yeast systems.

On May 15, the Proceedings of the National Academy of Sciences (PNAS) published an online paper on the research paper of the WangDong Research Group of the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences, the Richard Dixon Research Group of the University of North Texas and the Jing-Ke Weng Research Group of the Massachusetts Institute of Technology, usa, and the Jing-Ke Weng Research Group of the Massachusetts Institute of Technology.
the paper, two catalyticly inactive charketone isosome enzymes (CHIL1 and CHIL2) were involved in and regulated the biosynthesis of flavonols in hops.
orthodonol sofol and demethyl teofol are important secondary metabolites produced by humulus lupulus L. estual adenos, which belong to isoprene-based charketone compounds.
such compounds not only affect the flavor and quality of beer, but also have anti-cancer, antioxidant, regulate the body's metabolic balance and other important pharmacological effects, is currently found to be the most effective phytoestrogens.
in the study, the authors found that CHIL2 was able to interact with the upper and lower endofal protein charketone syntinase (CHS) and membrane-positioned opiate-based transferases (PT) in the synthetic pathway of flavonols, and their activity, forming CHS/PT/CHIL2 The metabolic complex can effectively catalyze the biosynthesis of flavonols, while HlCHIL1 can combine intermediate compounds such as grapefruit-based charketone and demethyl flaxrol in the metabolic pathway of flavonol to stabilize the open-ring structure of these compounds and maintain their physiological activity.
the authors further found that the function of CHIL2 binding and improving CHS enzyme activity is conservative in all terrestrial plants, suggesting that it has played an important role in the evolution of plants from aquatic to terrestrial.
the feasibility of using yeast system to produce stalcronic alcohol efficiently, the study has also applied for/obtained 6 patents.
these efforts provide the basis for the future use of industrial yeast to produce hop-specific terpenes for chassis, which in turn will produce "high value-added" beers that are healthy for the human body.
the first author of the paper is The Wang Dong Research Group PhD student Ban Zhaonan, Professor Richard Dixon of the University of North Texas and Researcher Wang Dong of the Institute of Genetic Development are co-authors.
this work is funded by the National Natural Science Foundation of China and the National Key Laboratory of Plant Genomics.
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