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The core of the production of bioenermaceutical energy from oil-producing microalgae lies in the efficient and low-cost obtaination of a large number of microalgae biomass.
At present, the indicators of the selection and breeding of oil-producing microalgae species mainly focus on its growth rate and biochemical group, but little attention is paid to the anti-wheelworm pollution ability, harvesting performance and other industrial characteristics of algae species, which are often the key to determine the success of the microalgae industrial culture system.
In view of the excellent easy-to-harvest characteristics of silky spirulina, the microalgae biotechnology team of Qingdao Institute of Bioenermaceuticals and Processes of the Chinese Academy of Sciences proposed a new idea in 2013 to "excavate oil-producing microalgae with excellent industrial characteristics from silky microalgae", completed the systematic evaluation of dozens of silky microalgae, and found for the first time that yellow silk algae cells (non-branched filaments) have high oil production capacity, resistance to oil and insect contamination and easy harvesting characteristics.
Under laboratory conditions, the content of yellow silk algae oil can reach 50 to 61%, of which neutral lipids account for 70 to 80%, algae cells can achieve more than 95% of direct airfly harvesting or direct filtration when no flocculant is added, open pool anniversary culture did not find obvious wheelworm contamination (Wang et al., 2013). the
study found that the accumulation of grease under photo-self-supporting conditions is not induced by nitrogen deficiency of the unique oil synthesis accumulation mechanism (Guo et al., 2014), through its oil metabolism pathway and key regulatory gene histological analysis and its response to environmental factors, identified the process of rapid accumulation of grease regulation methods (Wang et al., 2016).
studied the biodiesel conversion method of yellow silk algae oil and the conditions for the preparation of ethanol by hydrolyzing alginate, completed the 500 square meters scale of culture, harvesting, oil extraction and biodiesel conversion pilot, and established a process using yellow silk algae for biodiesel and fuel ethanol co-production (Wang et al., 2013; 2014).
study found that palm lysate (C16:1-9) in jaundice oil accounts for about 50 percent of total fatty acids, far higher than other common microalgae (Wang et al., 2016).
Palm lysate (C16:1-9) is an omega-7 monosaturated fatty acid, which, in addition to being as low as omega-3 fatty acids and beneficial to cardiovascular system health, is also considered to be a new hormone that can improve the body's sensitivity to insulin and play an important role in the prevention, health care and treatment of type 2 diabetes.
studies have found that yellow silk algae can use organic carbon sources, especially glucose for high density heterogenous growth characteristics (Wang et al., 2017; Zhou et al., 2017), through the optimization of organic carbon, nitrogen and phosphorus sources in the medium and inorganic nutrient salts, the yellow silk algae batch flow and fermentation process, the final biomass can reach 40g/L, the oil content is induced to reach about 45% dry weight.
technology has laid the foundation for the establishment of diesel fuel process for the production of omega-7 fatty acids in hetero-algae hetero-fermentation.
technology has filed Chinese invention patents and international PCT patents, has entered the United States, Japan, the European Union and other countries patent applications.
the above-mentioned research is supported by the National Support Plan Project, the "13th Five-Year Plan" project of the Chinese Academy of Sciences, the Demonstration City Project for Economic Innovation and Development of the State Oceanic Administration, and the Qingdao Science and Technology Program Project.
papers and patents: 1) Wang H, Gao LL, Chen L, Guo FJ, Liu TZ. Integration process of biodiesel production from filamentous oleaginous microalgae Tribonema minus. Bioresource Technology, 2013, 142:39-442) Guo FJ, Wang H, Wang JF, Zhou WJ, Gao LL, Chen L, Dong QZ, Zhang W, Liu TZ. Special biochemical responses to nitrogen features of filamentous oleaginous microalgae Tribonema sp. Bioresource Technology, 2014, 158:19-243) Wang H, Ji B, Wang JF, Guo FJ, Zhou WJ, Gao LL, Liu TZ. Growth and biochemicals make up of filamentous microalgae Tribonema sp.as potential feedstock. Bioprocess and Biosystems Engineering, 2014, 37:2607-26134) Wang H, Gao LL, Zhou WJ, Liu TZ. Growth and palmitoleic acidity of the complex of filamentous oleaginous microalgae Tribonema minus at varyings and light regimes. Bioprocess and Biosystems Engineering, 2016, 39:1589-15955) Wang H, Zhou WJ, Cheng WT, Gao LL, Liu TZ. Strategy study on enhancing lipid productivity of filamentous oleaginous microalgae Tribonema.Bioresource Technology, 2016, 218:161-1666) Wang H, Zhou WJ, Shao HM, Liu TZ. A comparative analysis of biomass and lipid content in five tribonema sp.strains at autotrophic, heterotrophic and mixotrophic. Algal Research, 2017, 24:284-2897) Zhou W, Wang H, Chen L, Cheng W, Liu T. Heterotrophy of filamentous oleaginous microalgae Tribonema minus for potential production of the lipid and palmitoleic acid. Bioresource Technology 239 (2017) 250-2578) Liu Tianjun, Chen Lin, Wang Hui, Wang Junfeng, Zhang Wei, Gao Lili, Zhou Wenjun. A method for preparing yellow silk algae bio-oil and the yellow silk algae bio - oil prepared by it. China Patent, Application No. 201310034308.3 (Authorized) 9) Zhou Wenjun, Liu Tianjun, Chen Lin, Wang Hui, Wang Junfeng, Gao Lili, Zhang Wei, Cheng Wentao. A new type of yellow silk algae and its culture and application. China Patent, Application No. 201510415879.010) Liu Tianjun, Zhou Wenjun, Wang Hui, Chen Lin. A method and application for the production of omega-7 fatty acid synthesis and cultured jaundice algae. PCT International Patent, Application No. PCT/CN2016/106587.