The function and mechanism of non-coding RNA SLERT in the transcription process of RNA polymerase I.

In a recent study, chen Lingling of the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences revealed the important functions and mechanisms of non-coding RNA SLERT in RNA polymerase I transcription.
may 5, the results of the study were published in cell, an internationally renowned academic journal.
long non-coding RNA, once thought of as the "dark matter" of the human gene, and many members of its family have been shown to be widely involved in the regulation of important life activities.
In this study, Chen Lingling's team used pre-created poly (A) tail RNA isolation and sequencing techniques to discover a new long non-coding RNA, the first long non-coding RNA found in human cells that regulates transcription of RNA polymerases.
the study also sheds new life on how this RNA differentiates, expanding the mechanism of action of long non-coding RNA.
nuclei are located in the nuclei of the cells and are important sites for RNA polymerase I transcription of RNA (rRNA) and rRNA processing.
erRNA transcription is the process of converting nuclear glycosome DNA (rDNA) into rRNA.
as the most in-cell RNA, rRNA transcription disorders are closely related to disease, insufficient transcription can lead to bone marrow failure anemia, and excessive transcription is prone to a variety of cancers.
new long non-coding RNA found in the study, which we named SLERT based on its structural properties and functions.
" Chen Lingling researchers said that the researchers used gene-editing technology to precisely knock out SLERT located in the nucleation of cells found that the absence of SLERT led to a decrease in RNA polymerase I transcription activity.
To further study the mechanism, the researchers observed that RNA lysogenase DDX21, present in cell nuclei, formed a ring-like structure with a diameter of about 400 nm around RNA polymerase I, in which RNA polymerase I is "surrounded" and its "enveloping ring" size directly affects the activity of RNA polymerase I transcription.
in-depth studies have shown that SLERT can change the protein composition of DDX21 in combination with DDX21 to adjust the size of the DDX21 ring.
the absence of SLERT can make the ring smaller leading to the function of RNA polymerase I is hindered, and when the ring becomes large, the DDX21 ring can be removed to inhibit RNA polymerase I.
human cells contain about 400 copies of the dna (rDNA) sequence, but only half can be converted into rRNA, what causes this rRNA transcription difference? Chen Lingling's research team also proposed a new mechanism for SLERT to control rRNA transcription differences by regulating RNA polymerase I transcription through the SLERT-DDX21 ring.
At the same time, the researchers also found that knocking out SLERT inhibited tumor growth in model mice, injected tumor cells that knocked out SLERT into mice, and the tumors in the body grew faster than mice injected into ordinary tumor cells, which also provided a new target for targeted treatment of related tumors.
experts believe that this study explains the molecular mechanism between protein, DNA and RNA in cell kernels, analyzes the regulatory mechanism of DDX21 ring for RNA polymerase I transcription, and the control effect of SLERT on DDX21 ring, reveals the new mechanism of RNA polymerase I transcription from a new perspective, and provides a new direction for further study of cell nuclease structure and function.
it is understood that the study was also greatly assisted by Yang Li researchers of the Chinese Academy of Sciences-Mapu Institute of Computational Biology, as well as the plant physiological ecology of cell analysis technology platform, biochemistry and cell analysis technology platform and animal experimental technology platform.
funding support comes from the Ministry of Science and Technology, the Fund Committee and the Chinese Academy of Sciences.
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