The grape germplasm resources and breeding team of Northwest A&F University used whole-genome sequencing to reveal the off-target effect of CRISPR/Cas9 on grapes

Original title: The grape germplasm resources and breeding team of Northwest A&F University used whole-genome sequencing to reveal the off-target effect of CRISPR/Cas9 on grapes

Recently, Horticulture Research published online a research paper entitled "Whole genome sequencing reveals rare off-target mutations in CRISPR/Cas9-edited grapevine" by Wang Xiping's research group from the grape germplasm resources and breeding team of Northwest A&F University

The study of the three wild-type (WT) and mutant 7 Cas9 grapes lines were whole gene sequencing analysis (Fig.

Grapes are widely planted around the world and are one of the important economic fruit trees

This research was funded by the National Natural Science Foundation of China (U1603234, 31572110, 32002000) and the Grape Germplasm Resources and Breeding Application Innovation Team Project (2013KCT-25)

Article link: https:// article is reprinted by "Food Science Network", and the article comes from Northwest Agriculture and Forestry University