The highest level of laying eggs on the back of chickens: preparation of active compounds by engineering bacteria

Biosynthesis of penicillin in yeast (http://biorxiv.org/content/biorxiv/early/2016/09/15/075325.full.pdf; doi: 10.1038/ncomms15202) Highlights: 1) the biosynthesis related genes of benzylpenicillin were assembled in yeast and successfully expressed; 2) Through promoter optimization, the yield of benzylpenicillin was increased; 3) a simple way to select the optimal promoter was established Figure 1: brief introduction of this article: antibiotics are widely used in clinic How to obtain such pure products quickly and efficiently is the original intention of this article In this paper, the penicillin family was used as the starting point to successfully heterologously express benzylpenicillin in the fungus with yeast as the substrate microorganism, and subcellular localization was carried out (as shown in Figure 1: A / B, strain name sc.p1); in order to improve the yield of benzylpenicillin, researchers replaced the promoters of one plasmid PCBC, pcla and Pende of strain sc.p1 to obtain a more high-yield strain sc.p2（ As shown in Fig 1: C); then, the promoters before PCBC, pcla and Pende were exchanged and optimized (as shown in Fig 1: D), so as to obtain three more high-yield yeast strains S5, S6, S7 In this paper, a large number of plasmids and their strains have been constructed For this purpose, researchers have established a set of activity-oriented, convenient and quick methods for screening dominant promoters (as shown in Figure 2), which provides convenience for subsequent research Figure 2: the percentage of establishment of S pyogenes growth from the screen (Orange) and the LCMS determined Benzylpenicillin yields (green) are shown for speed growth media from sc.p2 and 12 selected trains (from Natcom) extended reading: successful cases of heterologous expression such as artemisinin and lovastatin, bring gospel to patients and huge commercial value Although the yield of benzylpenicillin obtained in this paper is not high, as a positive start, it has opened up a way for yeast as a complex natural product (NRPS) of the chassis microbial biosynthesis of polynuclear fungi It is worth mentioning that this article was published in advance on the biological preprint website http://biorxiv.org/ last year, which is a matter of encouragement and pleasure The other is that Yang Sheng research group, the Key Laboratory of synthetic biology, Chinese Academy of Sciences, published the scientific research results entitled "iterative integration of multiple copy pathways in Yarrowia lipolytica for ergonomics β - carotene production" in "metallic Engineering" Through the strong promoter replacement and multi round copy number superposition of 11 coding genes of β - carotene heterologous synthesis pathway, 4 g / L β - carotene can be produced by the optimized medium supplement fermentation, and most of the products are stored in the intracellular oil drop This study shows that Yersinia lipolytica is an ideal cell factory for the production of hydrophobic terpenes Previous recommendations of the series of biosynthesis: 1 Illustrated documents @ chem SCI: laying eggs with chickens, expanding the capacity of lincomamide compound library by enzyme catalysis; 2 Illustrated documents @ NAT Chem bio magic hand, finding out the hidden compounds; 3 Illustrated documents @ PNAS Biosynthesis of anisomycin, a pyrrolidine protein synthesis inhibitor: a new step of gene integration and hidden biosynthesis 4: illustrated documents @ nature energy, I will save you with microorganisms! 5: new enzyme catalysis mechanism of @ JACS: formation of hydroxylamine note: due to the limited ability of the author, if there is any doubt above, please leave a message, we will contact you actively! Reprint please indicate: CBG information, Tao Xiaohua Welcome to the "CBG information" WeChat official account and QQ group: 498263368 (click join to join CBG- [biosynthesis]: https://jq.qq.com/ _wv=1027&k=49bs909, enter the secret number: Name + unit or research direction)