The identification and diagnosis of gene mutations is achieved by using DNA molecular regulation and synthesis of highly sensitive gold nanoparticles.

In living organisms, DNA guides the synthesis of proteins.
Ma Xingyi, a research professor at the Institute of Integrated Chemical Systems at Korea's Koryo University, first proposed that DNA molecules be used as skeletons to guide the synthesis of gold nanoparticles, and the results were published in 2016 in Nature Communications.
recently, Ma Xingyi and his team, together with scientists at Seoul National University and the University of Colorado in the United States, used DNA molecular regulation to synthesize pre-designed highly sensitive gold nanoparticles, enabling the identification and diagnosis of single-point mutations in breast cancer-related genes, the results were republished in Nature Communications and attracted the attention of the nanomaterials and medical engineering community.
, DNA Skeletons-Gold plasma nanomaterials are widely used in the field of optical sensing, and can interact with light and are highly sensitive and operable.
, gold nanomaterials are often used as substrate materials for biomedical sensing and imaging because of their unique stability and biocompatibility.
there was no liquid-phase synthesis method before, the precise preparation of the structure pre-designed gold nanoparticles.
gold nanoparticles have many structures, such as nanorods, nano-polyhedrons, nanochips, etc., but all of these structures are obtained by adding different auxiliary chemical molecules (such as CTAB), using trial and error, and gaining experience.
if you want to synthesize a spherical particle with a branch, there is nothing you can do about the above method, let alone synthesize a more complex, pre-designed structure.
this technical bottleneck has been addressed using intelligent molecular DNA.
Ma Xingyi and his team design the structure of DNA molecules, and then realize the regulation of the growth of gold nano, control accuracy of less than 5 nanometers, the entire synthesis process in aqueous solution, the synthesis of particles can be directly used in biomedical engineering, for the world's first.
reporters learned that in the latest study, the team will be THE DNA assembly between the two gold nano seeds, control the synthesis of the middle of the "bridge" or "gap" of the new gold nanoparticles, and the length of the bridge is only 2 nanometers, the light has a very high sensitivity, thus achieving the detection and identification of mutations in a point in the gene.
the "Gold- AND DNA Testing" gene mutation shas cause many diseases.
genes are huge, their structure is adaptive, and mutations at a certain site are difficult to detect.
however, the cells themselves can detect and repair genetic mutations, a mechanism that scientists discovered and won the 2015 Nobel Prize in Chemistry.
using this mechanism, the team used gene mismatch recognition proteins as an identification tool, using gold particles with nanobridges as sensing materials, reducing the entire sensing scale to nearly 30 nanometers.
for this sensing scale, biomolecules are no longer insignificant, and the very microscopic information of various molecules can be read in real time.
, the team analyzed and created a collection of data that could characterize the type of genetic mutation that could diagnose the genetic mutation in the sample under test.
Ma Xingyi graduated from Harbin University of Technology and is now a full-time research professor at the Institute of Integrated Chemical Systems at Gaoli University.
, the Institute of Integrated Chemical Systems at Koryo University is affiliated with the School of Chemical Engineering and Bioengineering, which ranks 43rd in the 2019QS World University.
Research Institute to technology industrialization and scientific research projects as the focus, Ma Xingyi's team is mainly responsible for the development of nano-biotechnology, specific research directions include (1) the use of biomolecules, the preparation of new optical nanomaterials for medical sensing and imaging, (2) the use of microfluidic chips to screen target cells, the use of cells for efficient biofermentation, the preparation of anti-tumor active substances, the main results were recognized by the Ministry of Science and Technology of Korea.
Source: Frontiers in Polymer Science.