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The Pleurotus ostreatus species complex is the most widely cultivated and very important medicinal fungus in China, and the commodity name is generally called "mushroom".
the individual morphology of this composite group is strong, vulnerable to environmental conditions and appear a variety of esophology, classification difficulties and confusion.
how to accurately construct the family tree of "mushroom" and identify the species quickly and accurately is a problem that must be solved in production.
have in the past identified mushroom species based on morphological features or sequences of individual DNA fragments, such as ITS, but this is difficult to achieve accurately.
Recently, Yang Zhuliang Research Group of Kunming Plant Research Institute of the Chinese Academy of Sciences carried out a systematic study of China's "mushrooms", collected 284 strains from various species preservation centers, mushroom growing enterprises and the wild, sequenced and analyzed four gene fragments (ITS, TEF1, RPB1 and RPB2), and clarified the inter-species relationship of cultivated mushrooms in China, combining the morphological characteristics to determine that these strains belong to 7 species. .abieticola, parsley side ear (apricot bowel) P.eryngii, Florida side ear approximate species P.cf.floridanus, (brown skin) side ear P.ostreatus, spore side ear P.placentodes, lung side ear P.pulmonarius and white side ear (white mushroom) P. tuoliensis; The evaluation concluded that RB2 was the most suitable DNA bar code for the identification of the composite group species in the four DNA fragments mentioned above.
common species of Chinese mushrooms: a. fir side ear Pleurotus abieticola; b. lung side ear P. pulmonarius; c. spore side ear P. placentodes; d. (brown skin side ear) P. ostreatus; e. The Florida Side Ear Approximate Species P.cf.floridanus study was funded by the National Key Basic Research Development Program (2014CB138305), and the academic paper was published on Mycological Progress under the title Speciess from Oyster Mushrooms in China and their DNA Barcoding.
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