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Flowering is an important sign that higher plants enter reproductive growth from nutrient growth, and is regulated by the double regulation of the gene that promotes or inhibits flowering.
FT/TFL1 gene family plays an important role in the flowering process of plants, in which the TFL1 (TERMINAL FLOWER 1) gene plays a role in inhibiting flowering.
Under the guidance of researcher Xu Zengfu, Li Chaoqiong, Ph.D. candidate of the Energy Plant Molecular Breeding Research Group of Xishuangbana Tropical Botanical Garden of the Chinese Academy of Sciences, and his co-authors conducted functional analysis of the three TFL1 gene JcTFL1a, JcTFL1b and JcTFL1c of the energy plant Jatropha curcas.
study found that the over-expression of JcTFL1b and JcTFL1c genes in wild-type amoeba and amoeba tfl1-14 mutants showed extreme late flowering, and that the flower sequence and fruit pods of GM amoeba showed abnormalities;
researchers further over-expression of three JcTFL1 genes in small tyb, the results showed that: 3 JcTFL1 gene over-expression of genetically modified small tyb showed a delayed flowering esoteric esoteric, and found that the genes promoting flowering JcFT and JcAP1 in the genetically modified plant significantly reduced expression.
addition, genetically modified small tybs silencing of the JcTFL1b gene obtained through RNA intervention technology showed early flowering.
the results show that the JcTFL1 gene is a flowering inhibitor gene of small tybss, which has the function of inhibiting plant flowering.
-modified small tygams that over-express the JcTFL1 gene show late flowering.
A, B and C charts are the super-expression of JcTFL1a, JcTFL1b, JcTFL1c genetically modified small tybs, E, F, G, respectively, corresponding to the stem-tip growth state, are in the nutritional growth.
D chart is wild-type small tseed, H is wild-type small tsego sequence.
results were published in Scientific Reports under the title Trree TFL1 homologues regulation floral institute in the biofuel plant Jatropha curcas.
the study was funded by the National Natural Science Foundation of China (31370595 and 31300568) projects.
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