the mechanism of lnc-ISG20 in the process of influenza virus infection and replication.

Influenza A influenza virus (Influenza A virus, IAV) belongs to the positive muscosal virus family, influenza virus genus.
the virus has a wide range of host species, can infect humans, birds and pigs and other species, has caused many world widespread epidemics, continue to human health, livestock and poultry breeding caused a major threat.
the process of infecting the host of viral infection can induce the body to produce a large number of long-chain non-coding RNAs (lncRNAs), how lncRNAs play a role in viral infection, how to regulate the host's anti-viral immune response, for the influenza virus cross-species transmission and viral disease prevention and control will be of guiding significance.
the current function of lncRNAs produced by host cells in influenza virus replication and the mechanism for regulating the natural immune response of antivirals are not clear.
recently, the Institute of Microbiology of the Chinese Academy of Sciences, Ye Wei group and Liu Wenjun team in collaboration with the influenza virus and host interaction research to achieve new results, following the previous discovery that ISG20 through the interaction with influenza virus NP protein and inhibit viral polymerase activity to inhibit viral replication, they recently found a long-chain non-coding RNA (lnc-ISG20) in the influenza virus infection and replication process playa key role.
by using RNA deep sequencing and other methods, the researchers screened a isg20 gene located at the same chromosome site and the influenza virus WSN/CA04 raised lncRNA (named lnc-ISG20), preliminary studies show that lnc-ISG20 is a interferon-induced gene, has the effect of suppressing influenza virus replication, and can raise isG20 protein levels.
in-depth studies have shown that in A549 cells treated with polymycysine (Poly I:C) and Sendai virus, the overexpression of lnc-ISG20 increases ISG20 protein levels, while lnc-ISG20 reduction reduces ISG20 protein levels.
, studies have shown that lnc-ISG20 inhibits IAV virus replication in a way that ISG20 relies on.
because lnc-ISG20 does not affect ISG20 mRNA levels, they speculate that lnc-ISG20 may promote ISG20 translation as a competitive endogenous RNA (ceRNA) for ISG20.
study found that miR-326 is a common miRNA of ISG20 and lnc-ISG20, which acts on 3'UTR of ISG20 mRNA and inhibits the translation of ISG20.
studies have confirmed that lnc-ISG20 can bind to miR-326, reducing the amount of miR-326 that binds to ISG20 mRNA.
in summary, the researchers demonstrated that lnc-ISG20 can be combined with miR-326 as a ceRNA, reducing its inhibitory effect on ISG20 mRNA, thereby increasing ISG20 protein levels and inhibiting influenza virus replication.
this study explains the action target of lncRNA-ISG20 regulating influenza virus and its molecular mechanism to inhibit influenza virus replication, and reveals the role of lnc-ISG20 as a new interferon-inducing gene in the host antiviral natural immune defense system.
doctoral student Chai Wenjia and associate researcher Jing Li are the first authors of the thesis, and researcher Ye Wei is the author of the article.
the research was funded by the National Key Research and Development Program and the National Natural Science Foundation of China.
the results were published online June 13 in the international academic journal Journal of Virology (Journal of Virology, 2018; doi: doi: 10.1128/JVI.00539-18).
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