The mechanism of membrane formation of Saf-mediated organisms of atypical bacteria of salmonella.

On November 10, the internationally renowned journal ELIFE published online the latest biofilm research paper "R.R.E. of the National Key Laboratory of Medical Genomics, " "The Construction of The Case of the Case and the recognition bio formation formation film byella Salmon Saf Pili" by The Research Paper of Ruijin Hospital, Shanghai Institute of Hematology, national key laboratory of medical genomics.
the paper reported on the mechanism of membrane formation of Saf-mediated organisms of salmonella atypical bacteria.
the author's elaboration of the mechanism of biofilm formation, or provides a new way of thinking to solve antibiotic resistance.
bacterial biofilm is a concentrated membrane sample formed by secreting a large amount of polysaccharides, proteins and nucleic acids to wrap itself in an extracellular matrix, in order to adapt to the environment, adhere to the surface of an object or human tissue.
biofilms are closely related to human health, such as causing the formation of plaque and infections in implantable medical devices.
compared to a single plankton bacteria, the formation of biofilms can produce stronger resistance to antibiotics and the host immune system, chronicizing and reproducing infections, leading to serious clinical problems.
, the mechanism of biofilm formation has always been the focus area of scholars at home and abroad.
Meng Guoyu team has long been committed to the study of the structural biology of blood diseases and pathogenic bacterial infections.
in biofilm research, the team 2011 in the world's top journal The EMBO Journal (European Organization for Molecular Biology) for the first time proposed the polymerization of pathogenic toxic protein Hap avicosin-mediated cells, leading to micro-colonies and the formation of biofilms.
it is understood that the research team this time focused on the "killer" salmonella hidden around us, the bacteria is responsible for large-scale food poisoning.
study lead actor Saf bacteria hair specific expression on most clinically pathogenic salmonella surfaces, consisting of a top SafD subkey connected to multiple SafA subquires.
research has found that Saf hairs have poly-adhesive (poly-adhesive activity) and self-polymering (self-associating activity) capabilities.
to understand the mechanism of Saf hair, the researchers obtained a single SafD and three continuous sub-SafDAA high-resolution protein structures using structural biology. By analyzing the structure of SafDAA protein cells,
boldly put forward the hypothesis that Saf bacteria hair can be mediated by the formation of a membrane by connecting the "handshake- oligopoly" at the beginning and end.
researchers used biophysics and cell function experiments to confirm the model from multiple angles.
previous findings combined with this study, it is a common phenomenon for bacteria to promote biofilm formation through intercellular glued molecular oligopoly.
researchers also found that pro20, which alters the protein's conformation through its specific pro-trans-conversion, affects the formation of biofilms.
note, proline is highly conservative in the links between other types of symas.
experts believe that this basic research work has potential application value.
one is to provide a fine field of view of target protein structure for salmonella prevention and treatment, such as vaccine and antibody development.
second, the author's elaboration of the mechanism of biofilm formation, for today's global antibiotic resistance to solve the problem, provides a new way to inhibit bacterial oligopoly (not to kill bacteria for the purpose).
Meng Guoyu's Ph.D. student Zeng Longhui, master's student Zhang Li and postdoctoral student Wang Pengran are the first authors of the article.
Professor Meng Guoyu is the author of the paper.
the research was supported by the National Natural Science Foundation of China, the Shanghai Science and Technology Commission Education Commission Fund and the Oriental Scholars Fund, as well as the Shanghai Synchrotron Radiation Light Source!