The media and basic characteristics commonly used in cell culture.

In cell culture, commonly used media and basic characteristics 1, RPMI-1640 MediumRPMI-1640 are widely used in mammals, special hematopoietic cells, normal or malignant hyperplhese white blood cells, hybrid tumor cell culture, is currently widely used in the medium.
are mainly used in suspended cell culture.
other lymphocytes such as K-562, HL-60, Jurkat, Daudi, IM-9, T-cell lymphoma cells, and HCT-15 epithelial cells can be used for reference.
2, Minimum Essential Medium (MEM), also known as the minimum required medium, contains only 12 essential amino acids, glutamine and 8 vitamins.
is simple and can be widely adapted to the culture of mammalian cell types that have been built and different places.
MEM-alpha is generally used to culture some difficult-to-culture cell types, while other cell lines with outthereings are almost always trained with MEM.
3, DMEM-high sugar (standard type) is a widely used medium, can be used in many mammalian cell culture, more suitable for high density suspended cell culture.
is suitable for poor adhesion, but do not want it to be separated from the original growth point of cloning culture, but also can be used in hybrid tumor myeloma cells and DNA transfection of conversion cells culture.
4, DMEM-low sugar (standard type) is a very widely used medium, can be used in many mammalian cell culture.
low sugar is suitable for dependent wall cell culture, especially for tumor cell culture with fast growth and poor adhesion.
5, DMEM/F12DMEM/F12 media suitable for the culture of cloning density.
F12 media components complex, containing a variety of trace elements, and DMEM in a 1:1 combination, called DMEM/F12 media (DME/F12) as the basis for the development of serum-free formulations, to take advantage of F12 contains more abundant ingredients and DMEM contains higher concentrations of nutrients as a benefit.
the medium is suitable for mammalian cell culture under low serum content.
in order to enhance the medium's buffering capacity, one of the improvements is the addition of a 15mMHEPES buffer to The DMEM/F12 (1:1).
6, McCoy's 5AMcCoy's 5A Medium is mainly designed for the culture of sarcoma cells to support the growth of a variety of primary transplants (e.g. bone marrow, skin, lungs, and spleen, etc.), in addition to suitable for general primary cell culture, mainly for tissue biopsy culture, some lymphocyte culture and some difficult-to-cultivate cell growth support.
such as Jensen rat sarcoma into fibroblasts, human lymphocytes, HT-29, BHL-100 and other epithelial cells.
7, Iscove's Modified Dulbecco (IMDM) Guilber and Iscove have improved Dulbecco'S Medium to Iscove's for the culture of red blood cells and macrophage precursors.
this culture contains selenium, extra amino acids and vitamins, sodium acetone acid and HEPES.
replaced iron nitrate with potassium nitrate.
IMDM also promotes the growth of Mouse B lymphocytes, LPS-stimulated B cells, bone marrow hematopoietic cells, T-cells and lymphoma cells.
IMDM is a very nutrient-rich culture, so it can be used for rapid proliferation culture of high density cells.
8, M-1991 Medium 1950, Morgan successfully developed a identified chemical composition of the cell culture liquid, known as M-199, mainly used in chicken embryo fibroblast culture.
this culture liquid must be supplemented by serum to support long-term culture.
M-199 can be used to grow cells from a variety of genus sources and to culture transfected cells.
9, Leibovitz Medium (L-15) L-15 culture solution is suitable for the culture of rapid proliferative tumor cells for the cultivation of tumor cell strains in the absence of CO2.
the culture is a phosphate buffer system, amino acid composition is further improved, and the semi-lactose replaces glucose.
10, Ham's F-10 medium: adapted to the culture of mouse cells and human diphmy.
11, Ham's F-12 media: can be applied with the addition of very few serums, especially suitable for single-cell culture and cloning culture, is a serum-free culture commonly used in the basic culture.
12, William's Medium E: Long-term cell culture for the epithelial cells of the liver in rats.
13, MCDB 131 culture: used to culture endothelial cells.
14, Opti-MEM I Reduced Serum Media: Used in the cultivation of hematopoietic cells.
15, plant hemagglutinin (PHA): Increases cell transformation and DNA synthesis.
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