The method of determining the activity of protease.
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Last Update: 2020-10-28
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Source: Internet
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Author: User
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protease
activity determination method: to the strain protease activity determination, do not need purification, what is the appropriate method? At the same time, it is introduced how to configure the fermentation and culture
the
as the substrate during the determination. Because casein is difficult to dissolve.
: You have to
heating
while tuning PH, alkaline casein will melt. It is easily dissolved by ammonia or a thin solution of sodium hydroxide.
: Protease determination is basically a classic method, that is, tyrosine as the standard curve of the method. This is my method of measuring protease, starting with tyrosine as the standard curve..
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