The molecular mechanism of rice protein quality formation is revealed

recently, Plant Cells published online the molecular mechanism of rice protein quality formation revealed by Wan Jianmin, a member of the Chinese Academy of Engineering and a researcher at the Crop Science Research Institute of the Chinese Academy of Agricultural Sciences. This study clones the new gene GPA5 for rice protein quality formation, and expounds the key role of GPA5 in the transport of gouldi after rice protein storage, which is of great significance to the improvement of rice protein quality.
, the first author of the paper and an associate researcher at the Institute of Agricultural Sciences, said rice contains a large amount of storage protein, which is the second largest group of nutrients in rice after starch. Gluten is the highest content of rice storage protein, accounting for more than 60% of the total protein content of rice, therefore, gluten is the preferred target for rice protein quality improvement. In endosperm cells, gluten is first synthesized in an endoennet in the form of a 57 kDa pregenuity, and then transported to the protein storage bubble by gourki to a dense vesicle-mediated way, under the action of liquid bubble processing enzymes, and eventually cut into mature gluten sub-base and storage. Little is known about the molecular regulatory mechanism of the capsule-mediated transport process.
the team discovered a new post-gluten gorsyl sub-selecting defect mutant gpa5, which confirmed by photo cloning that GPA5 encodes a plant-based regulatory factor with phospholipid binding. In endosperm cells, GPA5 is specificly distributed on the periphery of dense cysts. Subcellular positioning analysis confirmed that the membrane positioning of GPA5 depends on the pre-identified regulatory factors GPA1/Rab5a and GPA2/VPS9a. Biochemic analysis further confirms that GPA5 can be specific to the activation form of GPA1/Rab5a, indicating that GPA5 may be the effect factor of GPA1/Rab5a. Subsequent functional studies have found that GPA5 can be combined with corvet, a bolted complex, and SNARE, a membrane fusion complex containing VAMP727, which eventually leads to the fusion of dense vesicles and protein storage fluid bubbles to complete the transport of gluten.
the team has long been working on molecular genetic fundamentals for improved rice protein quality, with the goal of analyzing molecular network pathways for gluten synthesis, transport and deposition.
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