The molecular mechanism of TRBs to recruit PRC2 to target genes by combining the telobox base sequence is revealed.

On April 26, the journal Nature Genetics published an online study paper entitled Telobox motifs recruit CLF/SWN-PRC2 for H3K27me3 deposition TSRb s in-Arabiss, in collaboration with the Franziska Turck Research Group of the Mapp Institute in Germany and zhang Yiss of the Center for Excellence in Molecular Plant Sciences of the Chinese Academy of Sciences/ Zhang Yiss of the Institute of Molecular Physiological Ecology.
The H3K27me3 epithelial modification mediated by the Polycomb-group proteins complex has important regulatory effects in the specific development of animal and plant tissues.
its specific recruitment mechanism is unclear, and although individual sites and synergistic transcription factors have been reported, it remains unclear whether there is a relatively broad-spectrum recruitment mechanism.
january 2016, the Franziska Turck research team and Zhang's research team published Plant Cell and PLoS Genetics articles, each independently from genetics and high-throughput data mining to reveal the potential role of telobox binding protein TRBs (Telomere Repeat Proteins) and telobox base sequence recruitment pcG.
based on this, and through further collaboration, the project reveals the molecular mechanisms of TRBs by combining telobox base sequences to recruit PRC2 to target genes.
compared to the reported PcG-specific recruitment factor, TRB is a protein family with a broad spectrum of pcG complexes.
trb1/2/3 mutations are highly similar to phenotypes and expression patterns of PcG strong mutants (Figure A).
in the three mutants, H3K27me3 has a large-scale rearrangement (Figure B), and the H3K27me3 downfall area overlaps with the TRB1 binding site and the telobox area is highly overlapping (Figure C, D). further experimental evidence
that TRB1-3 can interact with PCG's core catalytic enzymes CLF and SWN, and that the itist expression after the telobox mutation on the promoter of The common target gene SEP3 of TRB1 and PcG, along with the h3K27me3 level, and the import of TRB1 partially restores inhibition.
, TRBs first bind to the telobox base sequence, and then recruit H3K27me3 of the PRC2 protein-mediated target gene through the interaction between TRBs and CLF/SWN, thereby inhibiting the expression of the target gene (Figure F).
the research was mainly done by Zhou Yue, a postdoctoral fellow at the Map Institute in Germany, and Wang Yue, a ph.d. student at the Institute of Plant Ecology, both under the guidance of Franziska Turck and Zhang Yixuan.
related work has been funded by the NSFC project.
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