On September 14th, the international academic journal Nature Communications published the latest research results of the Liu Xiaolong Research Group of the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences and the Guo Fan Research Group of Huaxi Hospital of Sichuan University, "Med23 Alls akeeper gate of the myeloid of the myeloid of hematopoetic cells stem".
the results reveal the molecular mechanism that regulates the differentiation potential of the hematopoietic stem cells.
hematopoietic stem cells maintain the steady state of the hematopoietic system by regulating self-renewal and differentiation.
in emergency situations, hematopoietic stem cells in a resting state can be activated quickly to differentiate the various blood cells needed, and it is not clear how the process is regulated.
under the guidance of researchers Liu Xiaolong and Guo Fan, postdoctoral research work by Chen Xufeng and others reveals that transcription intermediary sub-ji Med23 plays a vital regulatory role in the differentiation potential of hematopoietic stem cells.
the hematopoietic system specifically knockout Med23 mice peripheral blood routine index abnormal, as shown in lymphocytes, blood cells and hemoglobin reduction. The results of the analysis of hematopoietic stem cells and hematopoietic precursor cells in
showed that Med23 was impaired in self-renewal function of hematopoietic stem cells after knockout, the increase of myelin precursor cells and the decrease of gonorrhea precursor cells, indicating that the hematopoietic stem cells missing Med23 were biased towards myelin differentiation and reduced self-renewal ability.
RNA-seq analysis results revealed that Med23-knocked hematopoietic stem cells expressed myelin-related genes in advance and inhibited the expression of dry-related genes and gonorrhea signature genes.
single-cell RNA-seq analysis results further showed that Med23 knockout mice had a significant increase in myelin preference stem cells, while gonorrhea preferred hematopoietic stem cells decreased significantly.
continuous 5-FU myelin removal treatment in mice, Med23 knockout mice were able to differentiate the meel cells more quickly and thus better resist this emergency.
the work reveals the molecular mechanism of Med23 to regulate the differentiation potential of hematopoietic stem cells, and clarifies the relationship between transcription regulation, activation of hematopoietic stem cells and myelin differentiation potential mediated by Med23.
the research was carried out by Liu Xiaolong Research Group and Guo Fan Research Group, and was supported by the Animal Experimental Technology Platform, Cell Analysis Technology Platform and Molecular Biology Platform of the Public Technical Service Center of The Institute of Biochemistry and Cell.
the work is supported by the "973" project of the Ministry of Science and Technology, the pilot B project of the Chinese Academy of Sciences, the National Natural Science Foundation of China, and the postdoctoral fund of China.
Source: Shanghai Institute of Life Sciences.