The mouse solitary female monoploid nourishing layer stem cell line was successfully established.

Genetic information about eukaryotes is present in the form of chromosomes in the nucleus.
there are two sets of chromosomes in the diploid cells, which is the presentation pattern of genetic information in mammals.
and haploid cells have only one set of chromosomes, which reduces the complexity of their genomes, facilitates the acquisition of recessive purities, and is a valuable genetic research tool.
2011 and 2012, the lone female and male monoploid embryonic stem cell lines in mice were successfully established.
since then, rat, crab-eating monkeys, and human-derived hapluic embryonic stem cell lines have also been established.
these important haploblasttypes types have been applied to the screening of functional genes and the establishment of animal models.
and embryonic stem cells, the nourishing layer stem cells are self-renewing the secondary progenitor cells, can differentiate into different types of nourishing layer cells in the placenta, and are the ideal in vitro model for studying placenta development, function and related disease pathogenic mechanisms.
however, the current gene editing efficiency of nourishing layer stem cells is very limited, which limits its wide application in genetic research and so on.
the research team of the Institute of Zoology of the Chinese Academy of Sciences successfully established the haploid trophohoblast stem cell line (Haploid trophohoblast stem, cells haTSC) from the isolated female monoplocy sac embryo by optimizing the culture system, and demonstrated that it can maintain genomic stability during in vitro culture. further research
found that they highly express the classic nourishing layer stem cell marker genes and are very similar to the diphther nourishing layer stem cells in gene expression patterns. More
more importantly, in vitro and in vivo experiments show that the monoploid nourishing layer stem cells can efficiently chimed into placental tissue and differentiate the various cell types of the nourishing layer spectrum, with broad potential for extra-embryo development.
in addition, the team demonstrated that haplo-nourishing layer stem cells can be used as an effective gene screening for genome-wide pure-form genetic mutant cell banks.
the establishment of solitary female haploid nourishing layer stem cells is conducive to the rapid and efficient acquisition of the nourishing layer stem cells of the pure gene mutation, which will provide great convenience for the study of the fate and differentiation of the nourishing layer spectrum in the placenta, especially the construction of functional units.
results were published on January 8, 2019 in cell Reports, an international academic journal.
the research was carried out by the Institute of Animal studies and the Institute of Stem Cell and Regenerative Medicine Innovation of the Chinese Academy of Sciences. Zhou Qi and Li Wei, researchers at the
Animal Institute, are co-authors of the thesis, and PhD students Cui Wei, Jiang Liyuan and assistant researcher Li Tianda are co-authors of the paper.
the research by the Chinese Academy of Sciences strategic science and technology pilot special and the Ministry of Science and Technology, fund committee and other funding.
Source: Animal Research Institute.