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Recently, the official website of the National Health and Medical Commission issued a notice: In order to further optimize the new coronavirus detection strategy and serve the needs of epidemic prevention and control, after research, the State Council’s comprehensive team of the joint prevention and control mechanism for the new coronavirus pneumonia epidemic decided to conduct nucleic acid testing.
Once the document was released, it attracted widespread attention from the industry and the media
Tool 1 In-Fusion Technology Helps Gene Cloning Quickly and Efficiently!
Traditional cloning methods often have problems such as low enzyme cleavage efficiency, low ligation efficiency of multiple fragments and large fragments, lack of directionality in blunt-end ligation, self-ligation of vectors, and cumbersome subcloning steps
brand | Code No. | product name | Packaging quantity |
Clontech | 638947 | In-Fusion ® Snap Assembly Master Mix | 10 Rxns |
Clontech | 638948 | In-Fusion ® Snap Assembly Master Mix | 50 Rxns |
Clontech | 638949 | In-Fusion ® Snap Assembly Master Mix | 250 Rxns |
Tool 2 HEK293 expression vector expresses recombinant protein efficiently!
To obtain a large amount of recombinant protein, it is necessary to select an appropriate expression vector
brand | Code No. | product name | Packaging quantity |
Takara | 3390 | pHEK293 Ultra Expression Vector I | 20 μg |
Takara | 3392 | pHEK293 Ultra Expression Vector II | 20 μg each |
pHEK293 Ultra Expression Vector I is a single plasmid that can express the target protein in large quantities after transfection into HEK293 cells
Tool 3 Efficient Xfect Plasmid Transfection Reagent
With the cloning method and the construction of a suitable vector, the next step is to transfect the plasmid into HEK293 cells
In addition, it also has the characteristics of low toxicity
High-efficiency, low-toxic Xfect transfection reagent ensures that your transfection experiments are no longer worry-free!
brand | Code No. | product name | Packaging quantity |
Clontech | 631317 | Xfect Transfection Reagent | 100 Rxns |
Clontech | 631318 | Xfect Transfection Reagent | 300 Rxns |
Tools for Rapid Purification of Four Monoclonal Antibodies
Takara's Capturem Protein A and Protein G series provide fast, high-throughput solutions for antibody purification
[Comparison of antibody purification methods between Capturem and traditional resins]
Click to watch [ Capturem Quick Operation Video ]
In order to facilitate everyone's better understanding, we have prepared a gene expression manual, which is convenient for everyone to quickly get started from PCR cloning to nucleic acid purification, prokaryotic expression, mammalian expression, etc.
Takara has always contributed to life science research, and is committed to working with everyone to create a better life together! Throughout the ages, every large-scale plague and disease inevitably leads to the demise of a large number of lives, and now with the development and progress of science and technology, we have proved to nature time and time again that human beings are strong and unyielding.
For more details, please click: