The new mechanism of the imprinting and seed sleep of the DOGL4 gene by the DNA demethylating enzyme ROS1.

On September 28th, the Proceedings of the National Academy of Sciences (PNAS) published online a research paper entitled DNA demethylase ROS1 negativeregulates the imprinting of DOGL4 and seed dormancy in
the study found a new mechanism for the imprinting and seed sleep of the DOGL4 gene in the DNA demethylating enzyme ROS1 negative regulation of the DOGL4 gene.
gene imprinting refers to an epigenetic phenomenon in which significant differences in expression occur between parental and primary genes.
in plants, the imprint expression of genes mainly occurs in the embryo milk.
amoeba contains four DNA demethylase, in which DME-specific expression is expressed in the central cells of female matchmakers, which regulates the genome imprinting of the embryo by demethylating.
However, it remains to be seen whether other, more widely expressed demethylases are involved in the regulation of genomic imprinting.
the study found that another DNA demethylate ROS1 was involved in the imprinting of the DOGL4 gene.
DOGL4 is the homologous gene that regulates the seed sleep gene DOG1, which is a parent incomplete imprintgene in the embryo milk.
compared to the parent almoste gene, doGL4's parent allele is partially inhibited by the higher methylation of the -1.0 kb region of the promoter, which is mainly mediated by RNA-mediated DNA methylation (RdDM).
although DME and PRC2 complexes, the key factors that regulate the expression of imprinting genes, are also involved in regulating the expression of DOGL4, they do not regulate the imprinting of the DOGL4 gene.
in the ROS1 mutant, the DOGL4 parent allele is supermethylated in the promoter region, including -1.0 kb and -0.5 kb, resulting in the expression of the parent allele gene being completely inhibited, while the initiator region of the parent allele remains at a low methylation level, which ultimately makes DOGL4 a complete parent imprint gene in the ROS1 mutant.
, ros1 negatively regulates the imprinting of the DOGL4 gene by demethyling the parent allele promoter.
In addition, the study found that DOGL4 is involved in negative-regulated seed sleep and response to shedding acid (ABA), and that its imprint expression in the embryo milk also contributes to the regulation of seed hibernation and ABA response.
ROS1 is negatively regulated seed sleep and ABA response by positive regulation OF DOGL4 expression, while the two homologous genes DML2 and DML3 of ROS1 are not involved in regulating DOGL4.
the results reveal new features and mechanisms for ROS1 in regulating gene imprinting and seed sleep.
Huang Chaofeng's Ph.D. graduates Zhu Haifeng, Ph.D. students Xie Wenxiang and Xu Dachao are the co-first authors of the paper, and Huang Chaofeng and Zhu Health are co-authors of the paper.
the research was funded by the Center for Plant Adversity Biology of the Chinese Academy of Sciences, the Jiangsu Distinguished Youth Fund and the Chinese Academy of Sciences Pilot Project B.
Source: Shanghai Institute of Life Sciences.