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Yang Yungui of the Precision Genomics Medical Key Laboratory of the Beijing Genomics Research Institute of the Chinese Academy of Sciences and the Yang Yungui Research Group of the Center for Genetic and Developmental Collaborative Innovation discovered the molecular mechanism of the code reader YTHDF3 regulation mRNA translation in the course of studying the m6A methylation modification binding protein regulation mRNA processing law.
study was published online recently in the journal Cell Research, using the title of Cytoplasmic m6A reader YTHDF3 promotes mRNA translation.
team screened the mutual proteins of YTHDF3 and YTHDF1 by series affinity and precipitation, and found that they interacted mainly with the 40S sub-base protein of the nuclear glycogen and the 60S large sub-base protein.
the GST pull-down experiment showed that YTHDF3 interacted with these icing glycosomes and small substases.
further new protein synthesis experiments have shown that YTHDF3 can facilitate the translation of mRNA, and that the reduction in translation levels caused by knocking down YTHDF3 can only be supplemented by wild type YTHDF3.
combined with transcription groups and photorelinking-RNA sequencing and bio-information technology, it is proved that YTHDF3 and YTHDF1 can affect their respective RNA binding capabilities, and YTHDF3 promotes the translation efficiency of transcripts combined with YTHDF3 and YTHDF1, revealing the synergecy between YTHDF3 and YTHDF1 in the translation process.
the same journal, YTHDF3, a professor at the University of Chicago, has developed research on the regulation of mRNA translation and stability.
These results illustrate the molecular mechanism of m6A reader YTHDF3 regulation of mRNA translation, provide a basis for further study of m6A's biological function and RNA oscic inheritance, and provide a new direction of metaphysical regulation for the study of molecular mechanisms associated with normal physiological (e.g. stem cell dry maintenance and differentiation) or abnormal pathological life activities (e.g. malignant tumors).
the study was supported by projects such as the Ministry of Science and Technology, the National Natural Science Foundation of China and the Chinese Academy of Sciences.
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