The new strategy improves the breeding efficiency of corn hybridization.

Original title: New strategy to improve the efficiency of hybrid breeding of corn
corn is a special corn with important edible and industrial value that produces branched-chain starch. In recent years, the increase of market demand for corn has promoted the improvement and research work of corn quality. Recently, Anhui Agricultural University, in collaboration with the Crop Science Research Institute of the Chinese Academy of Agricultural Sciences, published the latest research results online in the Journal of Crop Science. They obtained the kinesies and hybrids quickly by using the target-like targeted mutation strategy.
Cheng Beiyu, author of the paper and a professor at Anhui Agricultural University, told the China Science Daily that traditional return breeding takes a longer period of time, and in the trans-breeding of the pyrethroid gene wx, it is often brought into the chromosome fragments of the parent of the body, that is, the chain burden commonly referred to in breeding, thus severely restricting the breeding efficiency.
In order to solve this problem, the study used corn hybrid 35 as a subject, and obtained the parental self-interbreeding 35F and 35M Wx genes of hybrid 35, respectively, through the target character directional mutation strategy (DTM), and obtained the indation 35F and 35M self-intercourse system after less generational interbreeding and SNP chip-assisted screening. This was followed by 35 corn hybrids with the characteristics of slugs obtained through hybridization, which increased the type of wx gene and improved breeding efficiency.Liu Changlin, co-author of the
paper and an associate researcher at the Crop Science Research Institute of the Chinese Academy of Agricultural Sciences, said the study used ZC01, a strain with a genetic background of ZC01 and a CRISPR/Cas9 system, to edit the Wx gene of the active mutant subject material through a living gene to produce a genosytic sex, and then screened the mutant offspring for a high genetic background recovery rate, a negative genetically modified and wx gene mutation.
results showed that the average content of improved niche parent and its hybrid embryo milk branch starch was as high as 94.9%, and the corresponding average content of wild endosperm branch starch was 76.9%. Genome-wide SNP data analysis showed that the recovery rate of paternal and matriarchal genetic background was as high as 98.19% and 86.78%, respectively, and that between parent and matriarchal systems did not contain CRISPR/Cas9 systems.
field trials showed that the team created a family of slugs and their hybrids similar to their corresponding wild agro-agronomics. "Our work provides an example of the use of CRISPR/Cas9 systems in commercial hybrids with targeted improved genetic transformation difficulties, broadening the application of gene editing techniques." Cheng said.
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