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Unlike the popular CRISPR/Cas system, prokaryotic Argonautes (pAgos) are considered to be more flexible gene editing tools because they do not require sequence motifs adjacent to their functional targets
In May 2016, China Han Chunyu identified another Ago protein (named NgAgo) derived from Natronobacterium gregoryi that can bind to guide DNA at 37°C and room temperature to achieve gene editing
Although Han Chunyu’s first discovery of NgAgo can be used as a mammalian gene editing tool, it is still unclear, but Kevin V.
NgAgo shares the standard domain with other catalytically active pAgo, but it also contains a previously unrecognized single-stranded DNA binding domain (repA)
The results of this study prove the potential of NgAgo in gene editing and provide new insights into previous seemingly contradictory reports
Compared with CRISPR/Cas9, NgAgo has a series of advantages such as non-specific dependence on PAM sequence, less failure and off-target with DNA as a guide, low fault tolerance rate, and high efficiency
Original search: https://doi.