The percoll cell separation fluid is made.

(i) principlePercoll is a silicone particle enculated with ethylene pyridoxine. The osmotic pressure is very low (-lt;20mosm/kg H2O), the viscosity is also very small, can form up to 1.3g/ml density, the use of pre-formed density gradient can be low centrifugal force (200 to 1000g) in a few minutes to tens of minutes to achieve satisfactory cell separation results. The gradient formed by the low diffusion constant in Percoll is very stable. In addition, Percoll does not penetrate biofilms and is non-toxic to cells, so it is widely used to isolate cells, subcellular components, bacteria and viruses, and to isolate damaged cells and their fragments from intact living cells.(ii) operating methods and precautions 1. Different concentrations (density) percoll solution Preparation: 9 percoll and 1 serving of 8.5% NaCl or 1.5MPBS were mixed to achieve physiological osmosis pressure, and then diluted to the desired concentration with a physiological solution (0.85% NaCl or 0.15M PBS).