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Transcription regulatory factor (Transcription Factor) is an important regulatory protein in microorganisms, which takes a specific metabolite as a ligand, binds to the promoter region of the regulated protein and regulates the downstream gene, and the amount of the desired ligand is positively related to the regulatory action within a certain range.
based on the above principle, transcription regulatory factors in microorganisms can be designed to identify specific target metabolites (Biosensor) for high-flux screening of high-yielding strains of target metabolites.
the traditional rational design, this method has the advantages of short screening cycle and good sensitivity.
The protein expression system and microbial metabolism research team, led by Zhang Dawei, a researcher at the Tianjin Institute of Industrial Biotechnology of the Chinese Academy of Sciences, built a set of components that can respond to intracerine phenylalanine content using transcription regulatory factors and convert phenylalanine concentrations into fluorescent signals.
design of biosensors, metabolic pathway evolution and algorithm analysis The component further improves the sensitivity and response range of the target product through system optimization.
this component and strain domestication mutagenification technology, successfully screened to high-yielding phenylalanine engineering bacteria.
the whole genome analysis of high-yielding strains obtained through screening, the mechanism of high-yielding anti-reverse of strains is explained in terms of physiological biogenetics, path optimization and energy supply.
method provides a new way of thinking for metabolic path reconstruction, strain evolution screening and pathway mechanism analysis.
the study was supported by the National Natural Science Foundation of China and the Tianjin Natural Science Foundation, and the results have been published in ACS Synthetic Biology.
Yongfei, an assistant researcher at Tianjin Institute of Technology, is the first author of the paper.
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