The phosphate code for GPCR recruitment blockers was cracked.

The international cross-team led by Xu Huaqiang, a researcher at the Shanghai Institute of Pharmaceutical Research of the Chinese Academy of Sciences, successfully analyzed the crystal structure of rhodopsin and arrestin compounds and cracked the phosphorylation code responsible for turning off the GPCR conduction signal.
July 27, the study was published in the journal Cell.
function of life is embodied or performed by relying on the signaling code.
G protein conjunctive receptosis (GPCR) is the largest family of cell membrane surface receptors in the human body, through the G protein and the deterrent protein two main signaling path, assume the role of cell signal transdivation "signal soldier".
GPCR activates the G protein to send an "open" signal when stimulated by external signals.
the "off" signal comes from the phosphorylation code - once the GPCR tail is phosphorylated, the deterrent protein is activated and tightly binded to the compound, thereby shutting down the conduction signal.
therefore the identification and interpretation of GPCR phosphorylation code is an important scientific issue in the field of cell signal transmission.
it is understood that Xu Huaqiang led the cross-team in 2015 successfully analyzed the GPCR and deterrent protein complex on the basis of the complete composite structure, the structure of the tail high-resolution structure and phosphorylation mechanism to carry out research.
"We used the world's strongest X-ray laser to see clearly the tail structure information of composite crystals and to analyze the process of phosphate recruitment in their tails and binding to the deterrent protein.
" Xu Huaqiang likened the research process to layer decryption of life passwords, "In order to verify the universality of phosphorylation passwords, we tested 96% of the GPCR protein and found that 70%-80% of GPCR's "off" signals were controlled by phosphorylation passwords."
" finally through a series of verification of biological function verification, GPCR recruitment deterrent protein phosphorylation code to crack - GPCR through its tail amino acid phosphorylation recruitment and binding to the deterrent protein, and found that the password is universal for the entire GPCR protein set.
it is understood that major breakthroughs in structural biology are often closely related to the combination of synchrogenous radiation light sources and X-ray free electron lasers.
there are currently six such combinations in Germany, the United States, Japan, South Korea, Switzerland and Italy.
we look forward to our own significant technological infrastructure, such as soft X-ray and hard X-ray free electron lasers under construction and propulsion," he said.
," said Xu Huaqiang, "these big scientific platforms can provide scientists with more advanced and rich comprehensive experimental methods."
, the study was funded by the national "major new drug creation" major projects, 973, pilot projects and international projects and other funds.
cooperative research institutions include the University of Toronto, the Scripps Institute, the Desy Free Electron Laser Science Center in Germany, the Hamburg Ultra-Fast Imaging Center in Germany, the University of California, Los Angeles, the University of Southern California, Shanghai University of Science and Technology and vanderboy University.
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