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Recently, Zhou Huanbin of the Institute of Plant Protection of the Chinese Academy of Agricultural Sciences (hereinafter referred to as the Plant Protection Institute) published a paper entitled "Single base replacement technology for the use of CRISPR/Cas9-guided human-derived AID (cytosine deaminase) protein in rice for high-efficiency target genes (impact factor 8.827), optimizing and expanding the target gene monobase-oriented replacement technology and its application range in rice.
it is understood that Zhou Huanbin team successfully developed the rice single-base editing system rBE3 and rBE4 tool box, the realization of the base C. G to T. The replacement of A.
, gc editing is inefficient due to TC preference for all APOBEC1 (rat-sourcecy deaminase). in
, the team introduced the human-derived AID cytosine deaminase, developed the rBE5 and rBE9 upgraded systems, and successfully and efficiently achieved efficient fixed-point editing of multiple target genes in rice.
the system avoids the problem of APOBEC1 (rat-sourcecy disamase) on TC preference, has the same high editing efficiency for TC, AC, GC and CC, greatly expands the application of single-base editing technology in plants, and plays a significant role in rice functional genomics and modern molecular breeding research. Ren Bin, a visiting doctoral student at the
Plant Protection Institute, is the first author of this article, and Zhou Huanbin, a researcher, and Professor Lin Honghui are co-authors of this article.
this research has been funded by the National Key Research and Development Program, the Innovation Engineering of the Chinese Academy of Agricultural Sciences and the National Nature Fund.
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