The preparation of a commonly used physiological salt solution.

Appendix I The preparation of a commonly used physiological salt solution in vitro or estosome organs and
tissues
experiments should be carried out, the specimen should be as close to the environment in the body as possible to ensure its normal life and its functional activities. It requires the following conditions:
(1) osmotic pressure is the same as tissue fluid;
(2) has the appropriate proportion of various ions necessary to maintain the normal functioning of tissue organs;
(3)
acid and alkali
degrees is the same as plasma and has buffer capacity;
(4) nutrients, oxygen and temperature are the same as tissue fluid. This type of liquid is called a physiological salt solution.I. Preparation of commonly used physiological salt solutions
animal species have different compositions of their substitutes and different permeable pressures, so the physiological salt solutions used as substitutes are also different in composition. For example, the permeable pressure of amphibian body fluids is equivalent to 0.65% NaCI solution, the permeability pressure of warm-blooded animal body fluids is equivalent to 0.9% NaCI solution, and the permeability pressure of sea vivid object fluids is equivalent to about 3% NaCI solution.
the need for internal environmental components, such as oxygen and nutrients, in the tissues and organs of different animals. Amphibian tissue organs require less oxygen and nutrients than warm-blooded animals. The purpose of various experiments is to make various changes in the composition of different physiological salt solutions. Commonly used are: physiological saline, Ren's liquid, Le's liquid, Tai's liquid and so on.
(i) Commonly used physiological salt solution and its components
(ii) the use of raw liquid to make physiological salt solution 2, common use of physiological salt solution
Salt water, i.e. sodium chloride
serum
and other sodium chloride solutions, was applied 0.6% to 0.65% in cold-blooded animals and 0.85% to 0.9% in mammals.
Ringer is mainly used for frog heart perfusion and other cold-blooded animal experiments, Locke is used for mammals heart, uterus and its exosome experiments, perfusion must be before the use of oxygen 15min. Low calcium lesions (containing waterless CaCl20.05g) are used for ionospheric bronchial perfusion in the intestinal intestinal tract and guinea pigs. Tyrode is used in mammalian intestinal experiments.
in recent years, tissue extraction and cell
culture
often require the treatment of tissues or cells with pancreatic
protease
(trypsin) or
edta
(Ethylene diamine tetraacetic acid, Versene). Ca2 plus or Mg2 plus have an impediment to this, so in this case, it is commonly used without Ca2 plus Mg2 plus physiological salt solution. Tissues or cells treated with trypsin, commonly used as a nutrient solution. Moscona solution composition is:
NaCl8.00 g
KCl0.20 g
NaH2PO40.005 g
NaHCO31.1 00 g
Glucose 2.00 g
plus distilled water to 1000 ml
tissues or cells to be treated, the nutrients used are:
Na-2 Versenate 0.2 g
NaCl8.0 g
KCl0.2 g
Na2HPO41.15 g
KH2PO40.2 g
Glucose 0.2 g
plus distilled water to 1000 ml 3, physiological salt solution preparation precautions
Generally should be pre-test preparation, not suitable for too long, so as not to occur pollution or certain components
national and chemical
changes and affect the results of the experiment, or first the various components of the solution are separately made into a certain concentration of the base solution backup, with the time to proportionally take the base solution preparation. In the preparation of various physiological salt solutions should pay attention to the following points:
1. Because the physiological salt solution of phosphate and carbonate root negative ions easily react with calcium ions, the production of insoluble calcium white phosphate or calcium carbonate precipitation. Therefore, in the preparation of physiological salt solution, first mix other ion originals and add distilled water, and finally the dissolved calcium chloride solution stirred while slowly added, in order to prevent calcium salt precipitation.
2. Glucose should be added in the near term, and the physiological salt solution added to glucose should not last long to avoid bacterial contamination and turbidity.
.