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experimental purpose:
II,reagents and equipment
three, operation method:
test tube number | blank tube | standard tube | assay tube |
serum (mL) | 0 | 0 | 1.0 |
standard protein (mL | 1.0 | 0 | |
Distilled water (mL) | 2.0 | 1.0 | 1.0 |
4.0 | 4.0 | 4.0 |
2, shake well, 37 degrees C water bath 20min after the use of gradometer at 540nm wavelength color, with a blank tube zero point, measured the absorption of each tube.
3, calculation: