The quality identification and preservation of enzyme-labeled antibodies.

1.Enzyme activity and
antibody
-effect cost determination
using bidirectional immuno-diffusion (diluted antibody) and DAB-H2O2 color-showing reaction detection in the binding antibody property and immune precipitation line enzymatic response.2.Enzyme and IgG
(mg/ml) - OD403
IgG (mg/ml) - (OD280- OD402) ( 0.42) 0.62
of which 0.42 is the enzyme protein itself (0.3) and the increased OD value after binding to dialdehyde, 0.94 is the antibody protein and aldehydease binding, OD280 increased by about 6%, so multiplied by 0.94. Rabbit IgG's OD280 is 0.62 at 1.0.
IgG amount (mg/ml) of sodium periodiodate marker method s (OD280- OD402 s 0.3) s 0.94 s 0.62
marker rate s OD403 / OD280 s0.3-0.6, i.e. binding 1-2 HRP molecules on an antibody molecule. The marking rate can be greater than 0.6-0.9. OD403 / OD280 is 0.4, which is approximately equivalent to E/P , which is 1.0.
ase label antibody preservation
certified enzyme label antibody, because sulphides and laminated nitrides, etc. can be combined with enzymes, so that HRP infested, therefore, can not be used sulphides and laminated nitrides as
preservatives
, can be added to the same amount of 60% glycelycen, small components (0.5ml / branch), placed 4 degrees C to 30 degrees C preservation.
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