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    Home > Active Ingredient News > Drugs Articles > The reagent form of POCT and the solution of the automatic system for the preparation of lyophilized beads

    The reagent form of POCT and the solution of the automatic system for the preparation of lyophilized beads

    • Last Update: 2019-12-06
    • Source: Internet
    • Author: User
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    Biological reagent preservation is an important problem in the medical industry In terms of reagent storage form, most of nucleic acid POCT reagent cards / trays need to be stored at room temperature, which requires that chemical reagents cannot exist in liquid form, but must be dehydrated and exist in solid form In use, the buffer solution is used for subsequent reaction The common methods of reagent dehydration are: 1 Spot drying; 2 Lyophilized beads The method of point solution drying is also common in the production of in vitro diagnostic reagents For example, the point enzyme of blood glucose test paper is the enzyme solution that upgrades the micro level to the nano level, drops onto the test paper membrane or sample card through the high-precision micro point solution system, and then evaporates the water in the reagent by heating and drying The technology of point solution drying is suitable for biological diagnosis chips such as blood glucose test paper, but not for nucleic acid POCT reagent The lyophilized bead process is to drop the reagent into the ultra-low temperature liquid to form a specific sphere shape Liquid nitrogen is the common ultra-low temperature liquid, and the temperature is - 194 ° Liquid nitrogen, liquid nitrogen, is inert (not reacting with other substances), colorless, odorless, noncorrosive, nonflammable, extremely low temperature Its excellent characteristics and reasonable price determine that liquid nitrogen is an ideal choice for low-temperature refrigeration medium The reagent liquid drops into liquid nitrogen, solidifies into a small ball in a very short time, then puts the solid ball into the pre cooled freeze-drying machine, designs the freeze-drying curve, and completes the freeze-drying The lyophilized beads can maintain the activity of enzyme / protein to a large extent, and the lyophilized beads have loose network structure and rapid re dissolution After being sealed, the solid pellets can be stored and transported at room temperature, which greatly reduces the transportation cost and preservation time These outstanding advantages of lyophilized bead technology are not only suitable for POCT process, but also suitable for other production processes of biological products, cosmetics and other products requiring fresh-keeping, ensuring active substances, normal temperature storage and transportation In the past few years, this new technology has been applied rapidly The volume of lyophilized beads is generally between 1-100 μ L At present, one of the major technical difficulties for liquid nitrogen freeze-dried beads is point solution: 1 Micro point solution: generally, liquid nitrogen freeze-dried beads need micro point solution, and the common point solution quantity ranges from 1 μ L (1 mg) to 100 μ L It is difficult for the common pump that people are familiar with to keep the point liquid stable and consistent in such a small range 2 High precision spot solution: the freeze-dried beads have higher requirements on the precision and shape of spot solution When the laboratory is operating, it is difficult to control the accuracy and consistency due to the problems of manual consistency and simple equipment If the ordinary pump is used, the controllability and accuracy of the pump are not good when the micro point liquid is used, which is very easy to cause the shape and size of the small ball are different, the shape of the ball is not good, or the small ball explodes into multiple microspheres when it falls on the surface of liquid nitrogen When the amount of liquid is small, it can't form a ball with the same shape When the amount is large, it is easy to separate into many small balls If the shape and size of lyophilized beads are different, it will affect the subsequent sub loading to the reagent disk / card, detection process and detection results In order to solve the above-mentioned problem of micro fluid high-precision liquid point, the technical team of Guangzhou feisheng precision equipment Co., Ltd has been focusing on the research and development of micro (micro level and nano level) fluid control system since 2010, Based on microfluidic pump technology, ds228 desktop system suitable for laboratory / small-scale production and ds328 floor system suitable for large-scale production have been successively launched, with a small volume of as low as 1 microliter, which is Taiwan, Famous bioengineering companies and university research institutes in Shenzhen, Chengdu, Tianjin, Ningbo, Zhuhai, Shanghai, Xiamen and other regions have provided system solutions for semi-automatic and automatic production of liquid nitrogen lyophilized beads and become professional system suppliers in this application field Ds228 desktop liquid nitrogen lyophilized bead production system ds328 floor standing liquid nitrogen lyophilized bead production system ds228 and ds328 liquid nitrogen lyophilized bead system adopt the precise micro ceramic pump technology with the point liquid volume as low as 1 μ L, and the components contacting with the liquid medicine are sapphire grade ceramics, Teflon and 316 stainless steel materials, which are not It reacts with the liquid medicine, thus ensuring the activity and stability of the liquid medicine without pollution Ds228 and ds328 systems can quickly adjust the amount and speed of liquid point In addition, the company can customize a variety of special needles, which can achieve high efficiency liquid point, no drop drop in liquid point, no explosion when it points to liquid nitrogen, good circular effect of lyophilized beads, uniform shape and volume, and the same size, effectively solving the problem that lyophilized beads are easy to drop or The problems of blasting, inaccurate accuracy, poor ball forming effect and poor consistency Guangzhou feisheng company has a variety of specifications of micro ceramic pump to choose from, as low as 1 μ l to more than dozens of μ l point liquid volume range can achieve high-precision point liquid, point liquid precision as high as 0.5%, can meet the customer's various precise point liquid requirements Ds228 and ds328 system can realize automatic production The parameters of automatic production can be simply set through the display screen Ds228 and ds328 systems can be equipped with single channel or multi-channel micropump point liquid system Single channel micropump system is suitable for laboratory or small batch production, and multi-channel micropump system is suitable for batch production Ds228 and ds328 systems are designed with unique mechanism, which is convenient for quick collection and storage of lyophilized beads The system is equipped with integrated liquid nitrogen tank and optional safety liquid nitrogen filling device Ds228 and ds328 systems have the advantages of micro (1 μ L), high precision (0.5%), high efficiency (100-200ppm), high consistency of lyophilized ball, and automatic continuous production technology, providing production equipment for biochemical companies  
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