The role and mechanism of norovirus NS3 protein in norovirus RNA replication.

On December 13, Zhou Xi, a researcher at the Wuhan Institute of Virology of the Chinese Academy of Sciences, made new advances in norovirus replication and antiviral research, based on human Norovirus NS3 has rna Helicandanding Activities, published online in the Journal of Virology.
Norovirus genus is a positive-chain RNA virus, resulting in infectious diarrhea that is prevalent worldwide, infecting an entire age group, infecting about 684 million people each year, causing more than 1.1 million hospitalizations and up to 210,000 deaths (mainly children, the elderly and patients taking immunosuppressants), as well as up to $60 billion in economic losses.
there is no vaccine or antiviral drug for the noreavirus.
In the replication process of RNA virus, the virus-coded decyclases and RNA molecular partners are thought to play an important role in unlocking the RNA double strands produced by the replication process and reshaping the RNA conformation of the virus.
currently, most RNA viruses are found to encode their own decyclones or RNA molecular partners.
However, it is not known whether noroviruses or even cup-like viruses can encode decyclases or RNA molecular partners, which hinders our understanding of such important human virus replication mechanisms.
study, Zhouxi team found that norovirus NS3 protein also has ATP-dependent RNA de-acting function, as well as the function of RNA molecular companion that is not dependent on ATP; further research
found that a small molecule drug approved by the FDA for the treatment of severe muscle weakness syndrome inhibits the de-acting activity of norovirus NS3 and can effectively inhibit norovirus RNA replication at the cell level.
the study for the first time, norovirus and cup-like virus coding has RNA delactatase and molecular companion activity of viral proteins, revealing its role and mechanism in norovirus RNA replication, deepening the understanding of the mechanism of the replication of this important pathogen virus, but also for the development of antinovirus drugs to provide a new way of thinking.
research work has been supported by the Royal Society and the National Foundation Of the Newton Senior Scholars Fund, the National Outstanding Youth Science Fund, the National Youth 863 Project, the Chinese Academy of Sciences strategic leading science and technology special projects, etc.
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